Neural Stem Cells Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Zigova, Tanja. (Editor ), Sanberg, Paul R. (Editor ), Sanchez-Ramos, Juan R. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2002.
Edición:1st ed. 2002.
Colección:Methods in Molecular Biology, 198
Materias:
Acceso en línea:https://doi.org/10.1385/1592591868
Tabla de Contenidos:
  • Isolation and Culture of NSCs
  • Neural Differentiation of Embryonic Stem Cells
  • Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens
  • Isolation of Stem and Precursor Cells from Fetal Tissue
  • Olfactory Ensheathing Cells
  • Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium
  • Production of Immortalized Human Neural Crest Stem Cells
  • Adult Rodent Spinal Cord Derived Neural Stem Cells
  • Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood
  • Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers
  • Characterization of NSCs in VITRO
  • Analysis of Cell Generation in theTelencephalic Neuroepithelium
  • Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures
  • Assessing the Involvement of Telomerase in Stem Cell Biology
  • Detection of Telomerase Activity in Neural Cells
  • In Vitro Assays for Neural Stem Cell Differentiation
  • Electron Microscopy and Lac-Z Labeling
  • Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells
  • Fluorescence In Situ Hybridization
  • RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells
  • Differential Display
  • Cell Labeling and Gene Misexpression by Electroporation
  • Gene Therapy Using Neural Stem Cells
  • Modeling Brain Pathologies Using Neural Stem Cells
  • Utilization/Characterization of NSCs in Vivo
  • Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model
  • Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods
  • Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo
  • Immunocytochemical Analysis of Neuronal Differentiation
  • Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold
  • Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS
  • Optimizing Stem Cell Grafting into the CNS
  • Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos
  • Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells
  • Routes of Stem Cell Administration in the Adult Rodent.