Electron Microscopy Methods and Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Hajibagheri, M. A. Nasser. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
Edition:1st ed. 1999.
Series:Methods in Molecular Biology, 117
Subjects:
Online Access:https://doi.org/10.1385/1592592015
Table of Contents:
  • General Preparation of Material and Staining of Sections
  • Negative Staining of Thinly Spread Biological Particulates
  • Preparation of Thin-Film Frozen-Hydrated/ Vitrified Biological Specimens for Cryoelectron Microscopy
  • The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry
  • High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling
  • The Application of LR Gold Resin for Immunogold Labeling
  • Low-Temperature Embedding in Acrylic Resins
  • Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections
  • Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol
  • Electron Microscopic Enzyme Cytochemistry
  • In Situ Molecular Hybridization Techniques for Ultrathin Sections
  • Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study
  • Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy
  • Applications of Electron Microscopy for Studying Protein-DNA Complexes
  • X-Ray Microanalysis Techniques.