Immunocytochemical Methods and Protocols
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
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| Edition: | 2nd ed. 1999. |
| Series: | Methods in Molecular Biology,
115 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592592139 |
Table of Contents:
- Antibody Preparation
- Overview of Antibody Use in Immunocytochemistry
- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
- Purification of Antibodies Using Ion-Exchange Chromatography
- Purification of Antibodies Using Affinity Chromatography
- Purification of Antibodies Using Protein A-Sepharose and FPLC
- Conjugation of Fluorochromes to Antibodies
- Biotinylation of Antibodies
- Tissue Preparation for Light Microscopic Analyses
- Overview of Cell Fixatives and Cell Membrane Permeants
- Preparation of Frozen Sections for Analysis
- Processing of Cytological Specimens
- Processing of Tissue-Culture Cells
- Processing of Tissue Specimens
- Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections
- Light Microscopic Detection Systems
- Overview of Fluorochromes
- Direct Immunofluorescent Labeling of Cells
- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells
- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells
- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts
- TUNEL Assay for Apoptotic Cells
- Overview of Fluorescence Analysis with the Confocal Microscope
- Overview of Laser Microbeam Applications as Related to Antibody Targeting
- Overview of Fluorescence Photomicrography
- Overview of Antigen Detection Through Enzymatic Activity
- The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
- The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue
- Multiple Antigen Immunostaining Procedures
- ELISA on Attachment-Dependent or Suspension Grown Cells
- Use of Immunogold with Silver Enhancement
- Fluorescence Activated Cell Sorter (FACS)Analyses
- Overview of Flow Cytometry and Fluorescent Probes for Cytometry
- Tissue Disaggregation
- Indirect Immunofluorescent Labeling of Viable Cells
- Indirect Immunofluorescent Labeling of Fixed Cells
- Fluorescent Labeling of DNA
- Deparaffinization and Processing of Pathologic Material
- Assay for Phagocytosis
- Assay for Filamentous Actin
- Assay for Chemoattractant Binding
- Assay for Oxidative Metabolism
- Colloidal Gold Detection Systems for Electron Microscopic Analyses
- Fixation and Embedding
- Preparation of Colloidal Gold
- Conjugation of Colloidal Gold to Proteins
- Colloidal Gold/Streptavidin Methods
- Pre-Embedding Labeling Methods
- Postembedding Labeling Methods
- Applications for Nucleic Acid Localization by In Situ Hybridization
- Overview of In Situ Hybridization
- In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe
- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes
- In Situ RT-PCR and Hybridization Techniques
- Special Applications for the Clinical Laboratory
- Overview of the Clinical Immunohistochemistry Laboratory: Regulations and Troubleshooting Guidelines
- Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors
- Overview of Automated Immunostainers.