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02960nam a22002895i 4500 |
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978-1-59259-250-0 |
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20190618011611.0 |
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cr nn 008mamaa |
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100301s1999 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592592500
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| 024 |
7 |
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|a 10.1385/1592592503
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Calcium Signaling Protocols
|c edited by David G. Lambert.
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| 250 |
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|a 1st ed. 1999.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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| 300 |
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|a XIII, 359 p. 63 illus., 2 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 114
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| 505 |
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|a General -- Fluorescent Measurement of [Ca2+]c -- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 -- Measurement of [Ca2+]i in Cell Suspensions Using Indo-1 -- Specialist Measurement Systems -- Confocal Microscopy -- Measurement of Intracellular Calcium Concentration Using Confocal Microscopy -- Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i) -- Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR) -- Nonelectrophysiological measurement of Ca2+ channel activity -- Measurement of Ca2+ Entry Using 45Ca2+ -- Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding -- Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores -- Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells -- Measurement of Inositol(1,4,5)trisphosphate Using a Stereospecific Radioreceptor Mass Assay -- Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay -- Microinjection of myo-Inositol(1,4,5)trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells -- Photolysis of Caged Calcium Using a Low-Cost Flash Unit -- Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology) -- Continuous Fluorescent Monitoring of Cellular Calcium Fluxe -- Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators -- Specialist Measurement Techniques -- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry -- Measurement of Calcium and Movement in Heart Cells -- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations -- Measurement of Cytosolic-Free Ca2+ in Plant Tissue -- Ca2+ Sensitive Targets -- Assay and Purification of Calmodulin-Dependent Protein Kinase -- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
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|a Lambert, David G.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592592503
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