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05155nam a22002895i 4500 |
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978-1-59259-260-9 |
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20191027002538.0 |
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cr nn 008mamaa |
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100301s1998 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592592609
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| 024 |
7 |
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|a 10.1385/0896035212
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Hepatitis C Protocols
|c edited by Johnson Y. N. Lau.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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| 300 |
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|a XXI, 621 p. 171 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Medicine,
|v 19
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| 505 |
0 |
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|a Detection of Anti-HCV in Serum -- Detection of Anti-GOR Antibodies in HCV Infection -- Confirmation of HCV Antibodies by the Line Immunoassay INNO-LIA HCV Ab III -- Detection and Quantitation of HCV in Serum -- Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridization -- Detection of HCV RNA in Serum by Reverse Transcription Polymerase Chain Reaction (RT-PCR) -- The AMPLICOR® HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNA -- Quantification of HCV RNA in Clinical Specimens by Branched DNA (bDNA) Technology -- Quality Control of the Polymerase Chain Reaction -- Preparation of Genotype-Specific HCV RNA Transcripts for Assessing HCV Detection and Quantification Assays -- Detection of HCV in Liver Tissue -- Detection of HCV RNA in Formalin-Fixed, Paraffin-Embedded Liver Tissue by RT-PCR -- Quantification of HCV RNA in Liver Tissue by bDNA Assay -- HCV Genotypes -- Hepatitis C Virus -- Molecular Evolutionary Analysis -- Genotyping by Type-Specific Primers That Can Type HCV Types 1–6 -- Genotyping Hepatitis C Virus by Type-Specific Primers for PCR Based on NS5 Region -- Determination of HCV Genotypes by RFLP -- HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II -- Serological Genotyping Using Synthetic Peptides Derived from the NS4 Region -- Determination of HCV Quasispecies by Cloning and Sequencing -- Detection of Hepatitis C Virus Quasispecies Heterogeneity by Single-Strand Conformational Polymorphism -- Hepatitis C Virus Heteroduplex Tracking Assay -- Detection of HCV In Situ -- In Situ Detection of HCV -- In Situ Detection of Hepatitis C Viral Antigens -- In Situ Hybridization and the Detection and Localization of HCV RNA -- The In Situ Detection of PCR-Amplified Hepatitis C RNA -- lmmunoelectron Microscopic Characterization of HCV -- Molecular Biologic Characterization -- Cloning and Assembly of Complex Libraries of Full-Length HCV cDNA Clones -- Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing -- Use of a Discistronic Vector for the Quantitation of HCV IRES Activity -- Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli -- Expression and Characterization of the HCV NS2 Protease -- Expression and Characterization of HCV NS3 Protease -- Expression and Characterization of the HCV NS3 Helicase Domain -- Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase) -- Detection and Molecular Cloning of the Extreme 3?-End of HCV -- In Vitro Stability of Hepatitis C Virus RNA -- Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome -- HCV-Specific Immunologic Response -- Role of Immune Response in HCV -- Determination of Hepatitis C Virus-Specific CD4+ T-Cell Activity in PBMC -- Measurement of HCV-Specific CD8+ Cytotoxic T-Cell Activities in the Peripheral Blood by Europium Release Assay -- Determination of HCV-Specific Bulk CD8+ Activity in Liver -- Characterization of HCV-Specific Cytotoxic T-Lymphocytes from Liver Tissue -- Production of Human Monoclonal Antibodies to Hepatitis C Virus and Their Characterization -- In Vitro Culture Model -- Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification -- Strand-Specific rTth RT-PCR for the Analysis of HCV Replication -- Cell Culture Systems for the Detection of HCV Infection -- Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines -- Primary Human Hepatocyte Culture for the Study of HCV -- A Cultivation Method for Highly Differentiated Primary Chimpanzee Hepatocytes Permissive for Hepatitis C Virus Replication -- Construction of Recombinant Viral Vectors for the Expression of HCV Genes -- Construction of Recombinant Vaccinia Virus Expressing HCV Genes -- Construction of Recombinant Adeno-Associated Virus (AAV) -- Production of Replication-Deficient Adenovirus Recombinants -- Generation of Recombinant Herpes Simplex Virus Ampiicons -- Construction of Recombinant Sindbis-Based Expression Vectors for the Study of HCV Genes and Their Products -- In Vivo Models -- The Chimpanzee Model -- Liver Transplantation as a Model to Study Hepatitis C Virus Infection.
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| 650 |
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|a Infectious diseases.
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| 650 |
1 |
4 |
|a Infectious Diseases.
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| 700 |
1 |
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|a Lau, Johnson Y. N.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
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|u https://doi.org/10.1385/0896035212
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