Peptide Research Protocols Endothelin /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Maguire, Janet J. (Editor ), Davenport, Anthony P. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2002.
Edición:1st ed. 2002.
Colección:Methods in Molecular Biology, 206
Materias:
Acceso en línea:https://doi.org/10.1385/1592592899
Tabla de Contenidos:
  • Endothelin Peptide Protocols
  • Immunocytochemical Localization of Endothelin Peptides, Precursors, and Endothelin-Converting Enzymes
  • Analysis of Endothelins by Enzyme-Linked Immunosorbent Assay and Radioimmunoassay
  • Separation of Endothelin Isoforms by High Performance Liquid Chromatography and Detection by Radioimmunoassay
  • Endothelin Receptor Protocols
  • Radioligand binding assays and quantitative autoradiography of endothelin receptors
  • Using Receptor Antagonists in Binding Studies to Characterize a Mammalian Endothelin Receptor
  • Quantification of Endothelin Receptor mRNA by Competitive RT-PCR
  • Endothelin-Converting Enzyme Protocols
  • Detection and Assessment of Endothelin-Converting Enzyme Activity
  • Quantitative Measurement of mRNA Levels by RT-PCR
  • Evaluation of Endothelin-Converting Enzyme Inhibitors Using Cultured Cells
  • Endothelin-Converting Enzyme Activity in Vascular Smooth Muscle Preparations In Vitro
  • In Vitro Functional Assays
  • Characterization of a New Endothelin Receptor Ligand by In Vitro Assays
  • Use of In Vitro Organ Cultures of Human Saphenous Vein as a Model for Intimal Proliferation
  • In Vivo Functional Assays
  • Investigation of the Endothelin System in Experimental Heart Failure
  • Local Forearm Vasoconstriction to Endothelin-1 Measured by Venous Occlusion Plethysmography In Vivo.