Opioid Research Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Pan, Zhizhong Z. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2003.
Edición:1st ed. 2003.
Colección:Methods in Molecular Medicine, 84
Materias:
Acceso en línea:https://doi.org/10.1385/1592593798
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490 1 |a Methods in Molecular Medicine,  |v 84 
505 0 |a Molecular Characterization of Opioid Receptors and Signaling Pathways -- Molecular Cloning of Opioid Receptors by cDNA Library Screening -- Expression of Opioid Receptors in Mammalian Cell Lines -- Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cells -- Analysis of Opioid-Induced Kinase Activation -- Study of Opioid Receptor Phosphorylation Using Cell-Labeling Method with 32P-Orthrophosphate -- Opioid Receptor Coupling to GIRK Channels -- Identification of Alternatively Spliced Variants from Opioid Receptor Genes -- Mapping and Detection of Endogenous Opioids -- Immunohistochemical Localization of ?-, ?- and K-Opioid Receptors Within the Antinociceptive Brainstem Circuits -- In Situ Hybridization in Neural Tissues -- Quantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping Genes -- Gene Arrays and Proteomics -- Model Systems for Studies on Opioid Functions -- Opioid Receptor Oligomerization -- Recombinant Opioid Receptors -- Receptor Knock-Out and Gene Targeting -- Pharmacological Interventions at the Spinal Cord -- Opioid Tolerance in Adult and Neonatal Rats -- Animal Models of Neuropathic Pain -- Place Conditioning to Study Drug Reward and Aversion -- Opiate Self-Administration -- Clinical Applications of Opioids -- Acute, Chronic, and Cancer Pain -- Clinical Treatment of Opioid Addiction and Dependence. 
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