p53 Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Deb, Sumitra. (Editor), Deb, Swati Palit. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2003.
Edition:1st ed. 2003.
Series:Methods in Molecular Biology, 234
Subjects:
Online Access:https://doi.org/10.1385/1592594085
Table of Contents:
  • Adenovirus Expressing p53
  • Purification of Recombinant p53 from Sf9 Insect Cells
  • Generation and Characterization of p53 Mutant Mice
  • Identification of p53 Target Genes by Fluorescent Differential Display
  • p53-Induced Gene Expression Analysis
  • Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Using SYBR® Green I
  • Transactivation and Transrepression Studies with p53
  • Methods to Study p53-Repressed Promoters
  • Interaction of p53 with Cellular Proteins
  • Interaction of p53 with the Adenovirus E1B-55 kDa Protein
  • In Vivo Footprinting and DNA Affinity Chromatography for Analysis of p53 DNA Binding Ability
  • Signaling to p53
  • Detection of Mitochondrial Localization of p53
  • Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress
  • Mutational Analysis of p53 in Human Tumors
  • Mutational Analysis of p53 in Human Tumors
  • Flow Cytometric Analysis of p53-Induced Apoptosis
  • Flow Cytometric Analysis of MDM2-Mediated Growth Arrest.