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03347nam a22002895i 4500 |
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978-1-59259-491-7 |
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20190617192627.0 |
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cr nn 008mamaa |
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100301s1988 xxu| s |||| 0|eng d |
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|a 9781592594917
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|a 10.1385/0896031276
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a New Nucleic Acid Techniques
|c edited by John M. Walker.
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| 250 |
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|a 1st ed. 1988.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1988.
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| 300 |
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|a XVI, 560 p. 17 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 4
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|a Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde -- Northern Blotting -- Hybrid-Release Translation -- Hybrid-Arrested Translation -- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells -- Use of Elutips to Purify DNA -- Plasmid Preparation on Sephacryl S1000 -- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels -- Use of 35S Nucleotides for DNA Sequencing -- Direct Dideoxy DNA Sequencing -- Computer Applications to Molecular Biology -- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique -- Oligonucleotide Synthesis Using the Manual Phosphotriester Method -- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method -- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis -- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries -- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors -- Cosmid Library Construction -- Construction of cDNA Libraries in ?gt10 or ?gt11 -- Immunoscreening of ?gt11 Expression Libraries Using an Avidin–Biotin Detection System -- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides -- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors -- Expression Screening of cDNA Libraries in pEX -- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins -- Epitope Mapping Using pEX -- DNA Transfection of Mammalian Cells Using Polybrene -- Expression of Foreign Genes in Mammalian Cells -- Dot-Blot Hybridization Method -- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes -- Photobiotin-Labeled DNA and RNA Hybridization Probes -- Enzyme-Labeled DNA Probes -- Luminescent Detection of Specific DNA Sequences -- Detection of DNA Sequences Using Biotinylated Probes -- Crosslinking of Single-Stranded DNA to Resins -- Extraction of RNA from Plants -- Isolation of Plant Nuclei -- In Vitro Transcription in Plant Nuclei -- Large-Scale Isolation of Ti Plasmid DNA -- Transformation and Regeneration of Dicotyledonous Plants -- Plant Protoplast Fusion -- Plant Tissue Culture -- Direct Gene Transfer into Plant Protoplasts -- Isolation of Wheat Chloroplasts and Chloroplast DNA -- DNA Labeling by Isolated Wheat Chloroplasts.
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| 650 |
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|a Biochemistry.
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| 650 |
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|a Biochemistry, general.
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| 700 |
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|a Walker, John M.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896031276
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