Practical Protein Chromatography

Bibliographic Details
Main Authors: Kenney, Andrew. (Author), Fowell, Susan. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1992.
Edition:1st ed. 1992.
Series:Methods in Molecular Biology, 11
Subjects:
Online Access:https://doi.org/10.1385/0896032132
Table of Contents:
  • Thiophilic Adsorption Chromatography
  • Immobilized Metal Ion Affinity Chromatography
  • Histidine Ligand Affinity Chromatography
  • Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes
  • Calcium-Dependent Hydrophobic Interaction Chromatography
  • Lectin Affinity Chromatography
  • Dye-Ligand Affinity Chromatography
  • High-Performance Affinity Chromatography for Protein Separation and Purification
  • Immunoafflnity Chromatography
  • Selection of Antibodies for Immunoaffinity Chromatography
  • Some Alternative Coupling Chemistries for Affinity Chromatography
  • Exploiting Weak Affinities
  • Biospecific Affinity Elution
  • Size-Exclusion High-Performance Liquid Chromatography of Proteins
  • Chromatofocusing
  • Ion-Exchange Chromatography of Proteins
  • Displacement Chromatography of Proteins
  • Purification of DNA Binding Proteins by Affinity and Ion Exchange Chromatography
  • Making Bacterial Extracts Suitable for Chromatography
  • Determination of Purity and Yield.