|
|
|
|
| LEADER |
03482nam a22003135i 4500 |
| 001 |
978-1-59259-518-1 |
| 005 |
20191027032649.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s1994 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781592595181
|
| 024 |
7 |
|
|a 10.1385/0896032582
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Protocols for Gene Analysis
|c edited by Adrian J. Harwood.
|
| 250 |
|
|
|a 1st ed. 1994.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1994.
|
| 300 |
|
|
|a XV, 411 p.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 31
|
| 505 |
0 |
|
|a Basic Recombinant Dna Techniques -- Transformation of Bacteria by Electroporation -- Direct Cloning of Agtll cDNA Inserts Into a Plasmid Vector -- PCR Cloning Using T-Vectors -- Thermal Cycle Dideoxy DNA Sequencing -- In Vitro Mutagenesis -- Ordered Deletions Using Exonuclease III -- Site-Directed Mutagenesis Using a Double-Stranded DNA Template -- Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template -- Construction of Linker-Scanning Mutations by Oligonucleotide Ligation -- Construction of Linker-Scanning Mutations Using the Polymerase Chain Reaction -- Localized Random Polymerase Chain Reaction Mutagenesis -- Genomic Structure -- The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells -- Physical Mapping of the Human Genome by Pulsed Field Gel Electrophoresis -- Field Inversion Gel Electrophoresis -- Enhanced Chemiluminescent Detection of Horseradish Peroxidase Labeled Probes -- Nonradioactive Oligonucleotide Probe Labeling -- Analysis of DNA Restriction Enzyme Digests by Two-Dimensional Electrophoresis in Agarose Gels -- Inverse Polymerase Chain Reaction -- Sequence Variations -- Use of Silver Staining to Detect Nucleic Acids -- A Nonradioactive Method for the Detection of Single-Strand Conformational Polymorphisms (SSCP) -- Temperature Gradient Gel Electrophoresis (TGGE) for the Detection of Polymorphic DNA and RNA -- TGGE in Quantitative PCR of DNA and RNA -- The PGK-PCR Clonality Assay (PPCA) -- Direct Sequencing of PCR Products -- Gene Expression -- The Use of Riboprobes for the Analysis of Gene Expression -- Quantification of Absolute Amounts of Cellular Messenger RNA by RNA-Excess Solution Hybridization -- Measurements of Rate of Transcription in Isolated Nuclei by Nuclear “Run-Off” Assay -- An RNA Polymerase II In Vitro Transcription System -- SI Mapping Using Single-Stranded DNA Probes -- Single Primer-Mediated Polymerase Chain Reaction -- Protein-Dna Interactions -- In Vivo DNA Footprinting by Linear Amplification -- DNA Photofootprinting with Rh(phi)2bpy3+ -- The Gel Retardation Assay -- The Southwestern Assay -- Cloning DNA Binding Proteins from cDNA Expression Libraries Using Oligonucleotide Binding Site Probes -- Protein Function -- 6xffis-Ni-NTA Chromatography as a Superior Technique in Recombinant Protein Expression/Purification -- Production of 35S-Labeled Proteins in E. coli and Their Use as Molecular Probes -- Preparation and Ligand Screening of a ?gt11 Lysogen Library.
|
| 650 |
|
0 |
|a Cell biology.
|
| 650 |
|
0 |
|a Biotechnology.
|
| 650 |
1 |
4 |
|a Cell Biology.
|
| 650 |
2 |
4 |
|a Biotechnology.
|
| 700 |
1 |
|
|a Harwood, Adrian J.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896032582
|