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03774nam a22002895i 4500 |
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978-1-59259-519-8 |
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20190618081835.0 |
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cr nn 008mamaa |
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100301s1994 xxu| s |||| 0|eng d |
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|a 9781592595198
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| 024 |
7 |
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|a 10.1385/089603268X
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Basic Protein and Peptide Protocols
|c edited by John M. Walker.
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| 250 |
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|a 1st ed. 1994.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1994.
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| 300 |
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|a XII, 490 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 32
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| 505 |
0 |
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|a The Lowry Method for Protein Quantitation -- The Bicinchoninic Acid (BCA) Assay for Protein Quantitation -- The Bradford Method for Protein Quantitation -- Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins -- SDS Polyacrylamide Gel Electrophoresis of Proteins -- Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins -- Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins -- Electrophoresis of Peptides -- Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels -- The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis -- Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dimension -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension -- Quantification of Proteins on Polyacrylamide Gels (Nonradioactive) -- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique -- Identification of Glycoproteins on Nitrocellulose Membranes and Gels -- The Release of Oligosaccharides from Glycoproteins -- Structural Profiling of Oligosaccharides of Glycoproteins -- Drying Gels -- Fluorography of Polyacrylamide Gels Containing Tritium -- The Electrophoretic Elution of Proteins from Polyacrylamide Gels -- Preparation of Proteins from Gels for Protein Microsequencing -- Comparison of Protein Primary Structures -- Protein Blotting -- Detection of Polypeptides on Immunoblots Using Secondary Antibodies or Protein A -- Detection of Proteins on Blots Using the Avidin-Biotin System -- Detection of Proteins on Protein Blots Using Chemiluminescent Systems -- The Detection of Proteins on Blots Using Gold or Immunogold -- Amino Acid Analysis -- Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography -- Peptide Purification by Reverse-Phase HPLC -- Enzymatic Methods for Cleaving Proteins -- Chemical Cleavage of Proteins -- Chemical Modification of Proteins -- The Dansyl Method for Identifying N-Terminal Amino Acids -- The Dansyl-Edman Method for Peptide Sequencing -- C-Terminal Sequencing of Peptides -- Analysis of Cysteine Residues and Disulfide Bonds -- Preparation and Characterization of Monoclonal Antibodies to Proteins and Other Cellular Components -- The Raising of a Polyclonal Antiserum to a Protein -- Production of Antisera to Synthetic Peptides -- Production of Antibodies Using Proteins in Gel Bands -- Preparation of Purified Immunoglobulin G (IgG) -- Enzyme and Biotin Labeling of Antibody -- Labeling of Peptides and Proteins by Radioiodination -- Radioimmunoassay of Peptides and Proteins -- Enzyme-Linked Immunosorbent Assay (ELISA) -- Free Zone Capillary Electrophoresis.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Walker, John M.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/089603268X
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