Immunocytochemical Methods and Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1995.
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| Edición: | 1st ed. 1995. |
| Colección: | Methods in Molecular Biology,
34 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/089603285X |
Tabla de Contenidos:
- Overview of Antibody Use in Immunocytochemistry
- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
- Purification of Antibodies Using Ion-Exchange Chromatography
- Purification of Antibodies Using Affinity Chromatography
- Purification of Antibodies Using Protein A-Sepharose and FPLC
- Conjugation of Fluorochromes to Antibodies
- Biotinylation of Antibodies
- Overview of Cell Fixation and Permeabilization
- Preparation of Frozen Sections for Analysis
- Processing of Cell-Touch Preparations
- Processing of Cytological Specimens
- Processing of Tissue-Culture Cells
- Processing of Tissue Specimens
- Overview of Fluorophores
- Direct Immunofluorescent Labeling of Cells
- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells
- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells
- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts
- Overview of Antigen Detection Through Enzymatic Activity
- The Peroxidase-Antiperoxidase (PAP) Method
- The Avidin-Biotin Complex(ABC) Method
- Labeled Avidin Binding (LAB) Method*
- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue
- ELISA on Attachment-Dependent or Suspension Grown Cells
- Use of Immunogold with Silver Enhancement
- Overview of Flow Cytometry and Fluorescent Probes for Cytometry
- Tissue Disaggregation
- Indirect Immunofluorescent Labeling of Viable Cells
- Indirect Immunofluorescent Labeling of Fixed Cells
- Fluorescent Labeling of DNA
- Deparaffinization and Processing of Pathologic Material
- Assay for Phagocytosis
- Assay for Filamentous Actin
- Assay for Chemoattractant Binding
- Assay for Oxidative Metabolism
- Fixation and Embedding
- Preparation of Colloidal Gold
- Conjugation of Colloidal Gold to Proteins
- Colloidal Gold/Streptavidin Methods
- Pre-Embedding Labeling Methods
- Postembedding Labeling Methods
- Overview of Fluorescence Photomicrography
- Overview of Fluorescence Analysis with the Confocal Microscope
- Overview of Laser Microbeam Applications as Related to Antibody Targeting
- Nucleic Acid Immunocytochemistry
- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes
- Overview of Automated Immunostainers
- Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors.