|
|
|
|
| LEADER |
02288nam a22002895i 4500 |
| 001 |
978-1-59259-541-9 |
| 005 |
20190618101631.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s1996 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781592595419
|
| 024 |
7 |
|
|a 10.1385/0896033139
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 100 |
1 |
|
|a Markie, David.
|e author.
|
| 245 |
1 |
0 |
|a YAC Protocols
|c by David Markie.
|
| 250 |
|
|
|a 1st ed. 1996.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1996.
|
| 300 |
|
|
|a XVIII, 372 p.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 54
|
| 505 |
0 |
|
|a Generation of Large Insert YAC Libraries -- YAC Library Storage and Transport -- YAC Library Screening I -- YAC Library Screening II -- Cloning of Human Telomeres in Saccharomyces cerevisiae -- Purification of YAC-Containing Total Yeast DNA -- Restriction Analysis of YACs -- RARE-Cleavage Analysis of YACs -- YAC Localization by Fluorescence In Situ Hybridization -- Alu-PCR Fingerprinting of YACs -- Detection of Chimerism in YAC Clones -- Amplification with Arbitrary Primers -- End Rescue from YACs Using the Vectorette -- Isolation of YAC Ends by Plasmid Rescue -- End-Rescue of YAC Clone Inserts by Inverse PCR -- Covering YAC-Cloned DNA with Phages and Cosmids -- Fragmentation and Integrative Modification of YACs -- Targeting Mutations to YACs by Homologous Recombination -- Reconstruction of Large Genomic Segments of DNA by Meiotic Recombination Between YACs -- Genomic Reconstruction by Mitotic Recombination of YACs -- Amplification of the Copy Number of YACs -- Transfer of YAC Clones to New Yeast Hosts -- Use of ACEDB as a Database for YAC Library Data Management -- YAC Transfer into Mammalian Cells by Cell Fusion -- YAC Transfer by Microinjection -- Transfection of Mammalian Cells via Lipofection -- The Isolation of cDNAs by Hybridization of YACs to cDNA Libraries -- cDNA Selection with YACs -- Markers, Selection, and Media in YAC Cloning.
|
| 650 |
|
0 |
|a Human genetics.
|
| 650 |
1 |
4 |
|a Human Genetics.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896033139
|