|
|
|
|
| LEADER |
03359nam a22002895i 4500 |
| 001 |
978-1-59259-544-0 |
| 005 |
20191027041851.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s1996 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781592595440
|
| 024 |
7 |
|
|a 10.1385/0896033325
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a In Vitro Mutagenesis Protocols
|c edited by Michael K. Trower.
|
| 250 |
|
|
|a 1st ed. 1996.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1996.
|
| 300 |
|
|
|a XIV, 391 p.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 57
|
| 505 |
0 |
|
|a Site-Directed Mutagenesis Using Positive Antibiotic Selection -- In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method -- Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates -- Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template -- Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach -- Analysis of Point Mutations by Use of Amber Stop Codon Suppression -- A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA -- Double-Stranded DNA Site-Directed Mutagenesis -- Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template -- Targeted Mutagenesis Mediated by the Triple Helix Formation -- A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site -- Ordered Deletions Using Exonuclease III -- Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis -- PCR-Based Site-Directed Mutagenesis -- In Vitro Recombination and Mutagenesis by Overlap Extension PCR -- Site-Directed Mutagenesis Using Overlap Extension PCR -- Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template -- Site-Directed Mutagenesis In Vitro by Megaprimer PCR -- Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids -- PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments -- Site-Directed Mutagenesis Using a Rapid PCR-Based Method -- A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Sequence -- A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered -- Multiple Site-Directed Mutagenesis -- Construction of Linker-Scanning Mutations by Oligonucleotide Ligation -- Construction of Linker-Scanning Mutations Using PCR -- Use of Codon Cassette Mutagenesis for Saturation Mutagenesis -- Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR) -- Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides -- Random Sequence Mutagenesis for the Generation of Active Enzymes -- Random Mutagenesis by Using Mixtures of dNTP and dITP in PCR -- PCR-Mediated Chemical Mutagenesis -- Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method -- An Efficient Random Mutagenesis Technique Using an E. coli Mutator Strain.
|
| 650 |
|
0 |
|a Cell biology.
|
| 650 |
1 |
4 |
|a Cell Biology.
|
| 700 |
1 |
|
|a Trower, Michael K.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896033325
|