PCR Sequencing Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1996.
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| Edición: | 1st ed. 1996. |
| Colección: | Methods in Molecular Biology,
65 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896033449 |
Tabla de Contenidos:
- Preparation and Analysis of DNA Sequencing Gels
- Purification of PCR Products from Agarose Gels for Direct Sequencing
- Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing
- Direct Sequencing of Double-Stranded PCR Products with the Sequenase Kit and [?-35 S] dATP
- Direct Sequencing by Thermal Asymmetric PCR
- Rapid Sequencing of cDNA Clones Direct Sequencing Using Sequential Linear/Asymmetric PCR
- Direct Sequencing of PCR Products Using Chemiluminescent Detection
- Direct DNA Sequencing of PCR Products Using Magnetic Beads
- Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products
- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
- Nonradioactive PCR Sequencing Using Digoxigenin
- Silver Sequencing™
- Direct Sequencing of PCR Products with DNA-Binding Proteins
- PCR Sequencing with the Aid of Detergents
- Direct Sequencing with Highly Degenerate and lnosine-Containing Primers
- Determination of Unknown Genomic Sequences Without Cloning
- DNA Sequencing by the Chemical Method
- Direct PCR Sequencing with Denaturants (Formamide)
- Efficient PCR Production of Single-Stranded DNA Sequencing Templates
- Preparation and Direct Automated Cycle Sequencing of PCR Products
- Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA
- Cloning PCR Products for Sequencing in M13 Vectors
- Sequencing PCR Products Cloned into M13 Vectors
- Genomic Amplification with Transcript Sequencing (GAWTS)
- DNA Rescue by the Vectorette Method
- Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations.