Lipoprotein Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Ordovas, Jose M. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1998.
Edition:1st ed. 1998.
Series:Methods in Molecular Biology, 110
Subjects:
Online Access:https://doi.org/10.1385/1592595820
Table of Contents:
  • DNA- and RNA-Based Methodolog
  • PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis
  • Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis
  • Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method
  • Generation of Transgenic Mice
  • Preparation of Knockout Mice
  • Protein-Based Methodology
  • Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins
  • Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography (FPLC)
  • Separation of Apolipoproteins by Polyacrylamide Gel Electrophoresis
  • Isolation and Purification of Serum Lipoprotein (a) Using Lectin Affinity
  • Electrophoretic Separation of LDL and HDL Subclasses
  • Measurement of Lipoprotein Particles
  • Methodological Approaches for Assessing Lipid and Protein Oxidation and Modification in Plasma and Isolated Lipoproteins
  • Separation and Quantitation of Phospholipid Classes by HPLC
  • Assays of Lecithin Cholesterol Acyltransferase (LCAT)
  • Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP)
  • In Vitro Measurement of Lipoprotein and Hepatic Lipases
  • Cellular Assays for Lipoprotein Receptors
  • Measurements of Proteoglycan-Lipoprotein Interaction by Gel Mobility Shift Assay.