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02831nam a22003015i 4500 |
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978-1-59259-604-1 |
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20190618011625.0 |
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cr nn 008mamaa |
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100301s1999 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592596041
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| 024 |
7 |
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|a 10.1385/089603612X
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Neurodegeneration Methods and Protocols
|c edited by Jean Harry, Hugh A. Tilson.
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| 250 |
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|a 1st ed. 1999.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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| 300 |
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|a XI, 306 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Medicine,
|v 22
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|a to Polymerase Chain Reaction -- Methodological Issues in Polymerase Chain Reaction for RNA -- Subtractive Hybridization of cDNA Libraries -- The Use of Differential Display RT-PCR for Identifying Altered Gene Expression in Ischemic Brain Injury -- Analysis of Gene Expression by Multiprobe RNase Protection Assay -- Molecular Probes for PNS Neurotoxicity, Degeneration, and Regeneration -- Mobility-Shift DNA-Binding Assay Using Gel Electrophoresis -- Transfection of Mammalian Cells In Vitro: Use in Analysis of Neuronal Damage -- Detection of Apoptotic Cells in the Nervous System -- Translocation Assays of Protein Kinase C Activation -- Astrocyte and Neuron Coculturing Method -- Viability Assays for Cells In Vitro: The Ethidium/Calcein Assay and the Immunofluorescence Combination Assay -- Measurement of the Nitric Oxide Synthase Activity Using the Citrulline Assay -- Measurement of Intraneuronal Free Calcium Using the Fluorescent Probe Technique -- Measurement of Calcium Buffering by Intracellular Organelles in Brain -- Receptor-Mediated Release of Inositolphosphates in Brain Slices -- The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins in Biological Functions -- Assays for the Analysis of Synaptic Proteins in Neurodegenerative Disorders -- Zymographic Method for the Measurement of Gelatinase Activity in Brain Tissue -- The Measurement of Monoamine Neurotransmitters in Microdialysis Perfusates Using HPLC-ECD -- Biochemical Measurement of Cholinesterase Activity -- Functional Study of Glutamate Receptor Channels in Brain Slices -- Selective Silver Staining for Neural Degeneration -- Immunohistological Markers of Cerebrovascular Integrity -- Electron Probe X-Ray Microanalysis: A Quantitative Electron Microscopy Technique for Measurement of Elements and Water in Nervous Tissue Cells.
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| 650 |
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|a Neurosciences.
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| 650 |
1 |
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|a Neurosciences.
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| 700 |
1 |
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|a Harry, Jean.
|e editor.
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| 700 |
1 |
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|a Tilson, Hugh A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/089603612X
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