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03145nam a22003015i 4500 |
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978-1-59259-679-9 |
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20191026202035.0 |
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cr nn 008mamaa |
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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592596799
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| 024 |
7 |
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|a 10.1385/1592596797
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Molecular Diagnosis of Infectious Diseases
|c edited by Jochen Decker, Udo Reischl.
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| 250 |
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|a 2nd ed. 2004.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XVI, 464 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Medicine,
|v 94
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| 505 |
0 |
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|a Protein-Based Identification Of Antigens -- Proteomic Approaches to Antigen Discovery -- Immunoproteomics -- Immunoprecipitation and Blotting -- DNA-Based Identification of Antigens -- Representational Difference Analysis of cDNA -- Microarray Data Analysis and Mining -- Expression Cloning -- Identification of Epitopes and Immunomodulatory Components -- Determination of Epitopes by Mass Spectrometry -- Identification of T-Cell Epitopes Using ELISpot and Peptide Pool Arrays -- Virus-like Particles -- Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components -- Expression of Recombinant Proteins -- High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format -- Production of Antigens in Chlamydomonas reinhardtii -- Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA -- Purification, Modification, and Renaturation of Recombinant Proteins -- Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies) -- Purification of Recombinant Proteins with High Isoelectric Points -- Refolding of Inclusion Body Proteins -- Small-Molecule-Protein Conjugation Procedures -- Characterization of Recombinant Proteins -- Structural Characterization of Proteins and Peptides -- Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors -- Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor -- Evaluation of Recombinant Proteins in Immunological Test Systems -- Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays -- Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays -- Basic Problems of Serological Laboratory Diagnosis -- Molecular Diagnostics Resources on the Internet -- Recombinant Receptor Molecules -- Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells.
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| 650 |
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|a Infectious diseases.
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| 650 |
1 |
4 |
|a Infectious Diseases.
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| 700 |
1 |
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|a Decker, Jochen.
|e editor.
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| 700 |
1 |
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|a Reischl, Udo.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592596797
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