Chromatin Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Becker, Peter B. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
Edición:1st ed. 1999.
Colección:Methods in Molecular Biology, 119
Materias:
Acceso en línea:https://doi.org/10.1385/1592596819
Tabla de Contenidos:
  • Expression and Purification of Recombinant Histones and Nucleosome Reconstitution
  • Preparation and Analysis of Positioned Nucleosomes
  • Site-Directed Chemical Probing of Histone-DNA Interactions
  • Base-Pair Resolution Mapping of Nucleosomes In Vitro
  • Equilibrium and Dynamic Nucleosome Stability
  • Nucleosome Structure and Dynamics
  • Analysis of Linker Histone Binding to Mono- and Dinucleosomes
  • Quantitative Analysis of Chromatin Higher-Order Organization Using Agarose Gel Electrophoresis
  • Analytical Ultracentrifugation of Chromatin
  • Analysis of Chromatin by Scanning Force Microscopy
  • In Vivo Mapping of Nucleosomes Using Psoralen-DNA Crosslinking and Primer Extension
  • Preparation of Chromatin Assembly Extracts from Xenopus Oocytes
  • Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos
  • A Solid-Phase Approach for the Analysis of Reconstituted Chromatin
  • Reconstitution and Analysis of Hyperacetylated Chromatin
  • Assembly of Mitotic Chromosomes in Xenopus Egg Extract
  • Nucleotide Excision Repair Coupled to Chromatin Assembly
  • Photolyase
  • Transcriptional and Structural Analyses of Isolated SV40 Chromatin
  • In Vitro Replication of Chromatin Templates
  • Analysis of HMG-14/-17-Containing Chromatin
  • Identification and Analysis of Native Nucleosomal Histone Acetyltransferase Complexes
  • Analysis of Nucleosome Disruption by ATP-Driven Chromatin Remodeling Complexes
  • Nucleosome Remodeling Factor NURF and In Vitro Transcription of Chromatin
  • An SDS-PAGE-Based Enzyme Activity Assay for the Detection and Identification of Histone Acetyltransferases
  • Analysis of DNaseI Hypersensitive Sites in Chromatin by Cleavage in Permeabilized Cells
  • Mapping of Nucleosome Positions in Yeast
  • Analysis of DNA Topology in Yeast Chromatin
  • DNA Methyltransferases as Probes for Chromatin Structure in Yeast
  • Restriction Nucleases as Probes for Chromatin Structure
  • Genomic Footprinting Using Nucleases
  • In Situ Analysis of Chromatin Proteins During Development and Cell Differentiation Using Flow Cytometry
  • Mapping DNA Target Sites of Chromatin Proteins In Vivo by Formaldehyde Crosslinking
  • Mapping DNA Interaction Sites of Chromosomal Proteins
  • UV Laser Footprinting and Protein-DNA Crosslinking
  • An In Vivo UV Crosslinking Assay That Detects DNA Binding by Sequence-Specific Transcription Factors.