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02607nam a22002895i 4500 |
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978-1-59259-789-5 |
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20190618021443.0 |
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cr nn 008mamaa |
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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592597895
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024 |
7 |
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|a 10.1385/1592597890
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
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|a Vaccinia Virus and Poxvirology
|b Methods and Protocols /
|c edited by Stuart N. Isaacs.
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250 |
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|a 1st ed. 2004.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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300 |
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|a XX, 396 p.
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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337 |
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|a computer
|b c
|2 rdamedia
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338 |
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|a online resource
|b cr
|2 rdacarrier
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490 |
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|a Methods in Molecular Biology,
|v 269
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505 |
0 |
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|a Working Safely with Vaccinia Virus -- Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers -- Construction of Recombinant Vaccinia Virus -- Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses -- Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA -- Construction of cDNA Libraries in Vaccinia Virus -- Construction and Isolation of Recombinant MVA -- Growing Poxviruses and Determining Virus Titer -- Rapid Preparation of Vaccinia Virus DNA Template for Analysis and Cloning by PCR -- Orthopoxvirus Diagnostics -- An In Vitro Transcription System for Studying Vaccinia Virus Early Genes -- An In Vitro Transcription System for Studying Vaccinia Virus Late Genes -- Studying Vaccinia Virus RNA Processing In Vitro -- Methods for Analysis of Poxvirus DNA Replication -- Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia Virus -- Pox, Dyes, and Videotape -- Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance -- Monitoring of Human Immunological Responses to Vaccinia Virus -- Vaccinia Virus as a Tool for Immunologic Studies -- Mouse Models for Studying Orthopoxvirus Respiratory Infections -- Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors -- Use of Dual Recombinant Vaccinia Virus Vectors to Assay Viral Glycoprotein-Mediated Fusion with Transfection-Resistant Primary Cell Targets -- Poxvirus Bioinformatics -- Preparation and Use of Molluscum Contagiosum Virus from Human Tissue Biopsy Specimens.
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650 |
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|a Microbiology.
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650 |
1 |
4 |
|a Microbiology.
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700 |
1 |
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|a Isaacs, Stuart N.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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856 |
4 |
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|u https://doi.org/10.1385/1592597890
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