Transmembrane Signaling Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Ali, Hydar. (Editor), Bodduluri, Haribabu. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2006.
Edition:2nd ed. 2006.
Series:Methods in Molecular Biology, 332
Subjects:
Online Access:https://doi.org/10.1385/1597450480
Table of Contents:
  • Overviews
  • Transmembrane Signaling by G Protein-Coupled Receptors
  • Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases
  • Protein-Protein Interactions in Signaling Cascades
  • Specific Topics
  • Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells
  • Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for the Pharmacological Characterization of CXCR4
  • Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors
  • G Protein-Coupled Receptor Dimerization and Signaling
  • Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cells
  • Isolation of Membrane Rafts and Signaling Complexes
  • Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae
  • Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions
  • Identification of Interacting Proteins Using the Yeast Two-Hybrid Screen
  • Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays
  • Clustering Assay for Studying the Interaction of Membrane Proteins with PDZ Domain Proteins
  • Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases
  • Affinity-Based Assay of Rho Guanosine Triphosphatase Activation
  • Assay of Phospholipase D Activity in Cell-Free Systems
  • Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipase D
  • Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors
  • Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo
  • Proteomic Analysis of Human Neutrophils.