Mycobacteria Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Parish, Tanya. (Editor ), Brown, Amanda Claire. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:2nd ed. 2009.
Colección:Methods in Molecular Biology, 465
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-207-6
Tabla de Contenidos:
  • Isolation of Mycobacterium Species Genomic DNA
  • Isolation of Mycobacterial RNA
  • Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization
  • Analyzing Lipid Metabolism: Activation and ?-Oxidation of Fatty Acids
  • Analysis of Lipid Biosynthesis and Location
  • Whole Genome Analysis Using Microarrays
  • Use of DNA Microarrays to Study Global Patterns of Gene Expression
  • Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria
  • Transport Assays and Permeability in Pathogenic Mycobacteria
  • Continuous Culture of Mycobacteria
  • Measuring Minimum Inhibitory Concentrations in Mycobacteria
  • Rapid Screening of Inhibitors of Mycobacterium tuberculosis Growth Using Tetrazolium Salts
  • Electroporation of Mycobacteria
  • Ins and Outs of Mycobacterial Plasmids
  • The Use of Temperature-Sensitive Plasmids in Mycobacteria
  • Heterologous Expression of Genes in Mycobacteria
  • Inducible Expression Systems for Mycobacteria
  • Assaying Promoter Activity Using LacZ and GFP as Reporters
  • Construction of Unmarked Deletion Mutants in Mycobacteria
  • Construction of Targeted Mycobacterial Mutants by Homologous Recombination
  • Phage Transposon Mutagenesis
  • Gene Essentiality Testing in Mycobacterium smegmatis Using Specialized Transduction
  • Gene Switching and Essentiality Testing
  • Insertion Element IS6110-Based Restriction Fragment Length Polymorphism Genotyping of Mycobacterium tuberculosis
  • Typing Mycobacterium tuberculosis Using Variable Number Tandem Repeat Analysis
  • Molecular Detection of Drug-Resistant Mycobacterium tuberculosis with a Scanning-Frame Oligonucleotide Microarray
  • MycoDB: An Online Database for Comparative Genomics of the Mycobacteria and Related Organisms
  • Environmental Amoebae and Mycobacterial Pathogenesis.