Membrane Trafficking

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Vancura, Ales. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edición:1st ed. 2008.
Colección:Methods in Molecular Biology, 457
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-261-8
Tabla de Contenidos:
  • Yeast Cells
  • 1 Freight Management in the Cell: Current Aspects of Intracellular Membrane Trafficking
  • High-Throughput Protein Extraction and Immunoblotting Analysis in Saccharomyces cerevisiae
  • Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays
  • A Cell-Free System for Reconstitution of Transport Between Prevacuolar Compartments and Vacuoles in Saccharomyces cerevisiae
  • In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptides
  • In Vitro Import of Proteins Into Isolated Mitochondria
  • Synthesis and Sorting of Mitochondrial Translation Products
  • In Vivo Labeling and Analysis of Mitochondrial Translation Products in Budding and in Fission Yeasts
  • Exploring Protein-Protein Interactions Involving Newly Synthesized Mitochondrial DNA-Encoded Proteins
  • Purification of Yeast Membranes and Organelles by Sucrose Density Gradient Centrifugation
  • Microscopic Analysis of Lipid Droplet Metabolism and Dynamics in Yeast
  • Use of Bimolecular Fluorescence Complementation in Yeast Saccharomyces cerevisiae
  • Mammalian Cells
  • Using Quantitative Fluorescence Microscopy to Probe Organelle Assembly and Membrane Trafficking
  • Measuring Secretory Membrane Traffic
  • A Correlative Light and Electron Microscopy Method Based on Laser Micropatterning and Etching
  • Approaches to Investigate the Role of Signaling in ER-to-Golgi Transport
  • Recruitment of Coat Proteins to Peptidoliposomes
  • SNARE-Mediated Fusion of LIposomes
  • Use of Polarized PC12 Cells to Monitor Protein Localization in the Early Biosynthetic Pathway
  • Tracking the Transport of E-Cadherin to and From the Plasma Membrane
  • Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes
  • Imaging pHluorin-Based Probes at Hippocampal Synapses
  • Analysis of Receptor Tyrosine Kinase Internalization Using Flow Cytometry
  • Measurement of Receptor Endocytosis and Recycling
  • Styryl Dye-Based Synaptic Vesicle Recycling Assay in Cultured Cerebellar Granule Neurons
  • Use of Quantitative Immunofluorescence Microscopy to Study Intracellular Trafficking: Studies of the GLUT4 Glucose Transporter
  • Dissecting GLUT4 Traffic Components in L6 Myocytes by Fluorescence-Based, Single-Cell Assays
  • Functional Genetic Analysis of the Mammalian Mitochondrial DNA Encoded Peptides.