Plant Signal Transduction Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Pfannschmidt, Thomas. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edition:1st ed. 2009.
Series:Methods in Molecular Biology, 479
Subjects:
Online Access:https://doi.org/10.1007/978-1-59745-289-2
Table of Contents:
  • Multiple Dimensions in Plant Signal Transduction: An Overview
  • Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions
  • Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis
  • Accurate Real-time Reverse Transcription Quantitative PCR
  • Probing Spatio-temporal Intracellular Calcium Variations in Plants
  • Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy
  • Imaging of Reactive Oxygen Species In vivo
  • Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single-Cysteine Mutant-Immobilized Resin
  • Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes
  • Examining Protein Stability and Its Relevance for Plant Growth and Development
  • Probing Circadian Rhythms in Chlamydomonas reinhardtii by Functional Proteomics
  • Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells
  • Fluorescence Cross-Correlation Spectroscopy of Plant Proteins
  • The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin system (mbSUS)
  • Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways
  • Phosphatase Activities Analyzed by in vivo Expressions
  • Chromatin Immunoprecipitation Experiments to Investigate In Vivo Binding of Arabidopsis Transcription Factors to Target Sequences
  • Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins
  • Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay
  • Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation
  • RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System.