Kidney Research Experimental Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Hewitson, Tim D. (Editor ), Becker, Gavin J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 466
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-352-3
Tabla de Contenidos:
  • Isolation and Propagation of Cell Populations
  • Isolation and Propagation of Glomerular Mesangial Cells
  • Isolation and Primary Culture of Human Proximal Tubule Cells
  • Propagation and Culture of Renal Fibroblasts
  • Animal Models
  • Small Animal Models of Kidney Disease: A Review
  • Techniques and Applications
  • Measurement of Glomerular Filtration Rate in Conscious Mice
  • Laser Capture Microdissection of Kidney Tissue
  • Quantitative Gene Expression Analysis in Kidney Tissues
  • In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy
  • Using In Situ Hybridisation to Localize Renal Gene Expression in Tissue Sections
  • Immuno and Lectin Histochemistry for Renal Light Microscopy
  • Immuno and Lectin Histochemistry for Renal Electron Microscopy
  • Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia
  • Identification of Apoptosis in Kidney Tissue Sections
  • Cell-Populated Floating Collagen Lattices: An In Vitro Model of Parenchymal Contraction
  • Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells
  • Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue
  • SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue
  • In Vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli.