Type 2 Diabetes Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Stocker, Claire. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 560
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-448-3
Tabla de Contenidos:
  • Selecting the “Right” Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research
  • Nutritional Models of Type 2 Diabetes Mellitus
  • The Isolation and Purification of Rodent Pancreatic Islets of Langerhans
  • The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans
  • The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ?-Cell Lines
  • In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function
  • Single-Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells
  • Laser Capture Microdissection of Human Pancreatic ?-Cells and RNA Preparation for Gene Expression Profiling
  • In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells
  • The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes
  • Pancreatic Remodeling: Beta-Cell Apoptosis, Proliferation and Neogenesis, and the Measurement of Beta-Cell Mass and of Individual Beta-Cell Size
  • Morphology of Pancreatic Islets: A Time Course of Pre-diabetes in Zucker Fatty Rats
  • Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas
  • The Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent
  • Hyperinsulinemic–Euglycemic Clamp to Assess Insulin Sensitivity In Vivo
  • Gene Expression Analysis in Diabetes Research
  • Gene Expression Mining in Type 2 Diabetes Research.