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03494nam a22003135i 4500 |
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978-1-59745-521-3 |
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20191027041305.0 |
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cr nn 008mamaa |
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100316s2009 xxu| s |||| 0|eng d |
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|a 9781597455213
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| 024 |
7 |
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|a 10.1007/978-1-59745-521-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Mitochondrial DNA
|b Methods and Protocols /
|c edited by Jeffrey A Stuart.
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| 250 |
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|a 2nd ed. 2009.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XII, 404 p. 74 illus., 1 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 554
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| 505 |
0 |
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|a Mitochondrial DNA Replication, Transcription, Translation, and Respiratory Complex Assembly -- Biochemical Isolation of mtDNA Nucleoids from Animal Cells -- Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis -- Comparative Purification Strategies for Drosophila and Human Mitochondrial DNA Replication Proteins: DNA Polymerase ? and Mitochondrial Single-Stranded DNA-Binding Protein -- Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease -- Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein -- Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA -- Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Helicase Twinkle -- Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase -- Methods for Studying Mitochondrial Transcription Termination with Isolated Components -- Oxidative Phosphorylation: Synthesis of Mitochondrially Encoded Proteins and Assembly of Individual Structural Subunits into Functional Holoenzyme Complexes -- Mitochondrial DNA Damage and Repair -- Reactive Oxygen Species Production by Mitochondria -- Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach -- Quantitative Analysis of Oxidized Guanine, 8-Oxoguanine, in Mitochondrial DNA by Immunofluorescence Method -- In Vitro Measurement of DNA Base Excision Repair in Isolated Mitochondria -- Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through Stable Transfection, Transient Transfection, Viral Transduction, and TAT-Mediated Protein Transduction -- Construction and Characterization of a Cell Line Deficient in Repair of Mitochondrial, but Not Nuclear, Oxidative DNA Damage -- Mitochondrial DNA Mutations -- Mitochondrial DNA Oxidative Damage and Mutagenesis in Saccharomyces cerevisiae -- Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysis -- Rapid Identification of Unknown Heteroplasmic Mitochondrial DNA Mutations with Mismatch-Specific Surveyor Nuclease -- Collection of Isolated Cells for Studying Mitochondrial DNA Mutations Within Individual Cells -- Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR -- Measuring DNA Precursor Pools in Mitochondria -- Establishment of Human Cell Lines Lacking Mitochondrial DNA.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Human genetics.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Stuart, Jeffrey A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-59745-521-3
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