Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Marx, Andreas. (Editor ), Seitz, Oliver. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edición:1st ed. 2008.
Colección:Methods in Molecular Biology, 429
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-60327-040-3
Tabla de Contenidos:
  • Basic Principles of Fluorescence and Energy Transfer
  • Applications
  • Multiplex Detection of Mutations
  • Detection of Salmonella Species in Foodstuffs
  • Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR
  • Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma
  • Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan® Real-time PCR and RT-PCR
  • High-Throughput Real-Time PCR
  • Design and Use of Scorpions Fluorescent Signaling Molecules
  • Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification
  • New Technologies
  • EasyBeacons™ for the Detection of Methylation Status of Single CpG Duplets
  • Quenched Autoligation Probes
  • HyBeacon® Probes for Rapid DNA Sequence Detection and Allele Discrimination
  • FIT-Probes in Real-Time PCR
  • SNP Genotyping by Unlabeled Probe Melting Analysis
  • Non-Nucleic Acid Targets
  • Molecular Beacons for Protein—DNA Interaction Studies
  • DNA Polymerase Profiling
  • Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions
  • Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes.