Structural Proteomics High-Throughput Methods /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Kobe, Bostjan. (Editor ), Guss, Mitchell. (Editor ), Huber, Thomas. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edición:1st ed. 2008.
Colección:Methods in Molecular Biology, 426
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-60327-058-8
Tabla de Contenidos:
  • Protein Target Selection, Bioinformatic Approaches, and Data Management
  • Target Selection for Structural Genomics: An Overview
  • A General Target Selection Method for Crystallographic Proteomics
  • Target Selection: Triage in the Structural Genomics Battlefield
  • Data Management in Structural Genomics: An Overview
  • Data Deposition and Annotation at the Worldwide Protein Data Bank
  • Prediction of Protein Disorder
  • Protein Domain Prediction
  • Protein Structure Modeling with MODELLER
  • Protein Production
  • High Throughput Cloning with Restriction Enzymes
  • Automated Recombinant Protein Expression Screening in Escherichia coli
  • From No Expression to High-Level Soluble Expression in Escherichia coli by Screening a Library of the Target Proteins with Randomized N-Termini
  • Application of High-Throughput Methodologies to the Expression of Recombinant Proteins in E. coli
  • A High Throughput Platform for Eukaryotic Genes
  • High Throughput Production of Recombinant Human Proteins for Crystallography
  • Assembly of Protein Complexes by Coexpression in Prokaryotic and Eukaryotic Hosts: an Overview
  • Cell-Free Protein Synthesis for Analysis by NMR Spectroscopy
  • A Medium or High Throughput Protein Refolding Assay
  • Structural Proteomics of Membrane Proteins: a Survey of Published Techniques and Design of a Rational High Throughput Strategy
  • Biophysical and Functional Characterization of Proteins
  • Methods for Protein Characterization by Mass Spectrometry, Thermal Shift (ThermoFluor) Assay, and Multiangle or Static Light Scattering
  • High Throughput Methods for Analyzing Transition Metals in Proteins on a Microgram ScaleHigh Throughput Methods for Analyzing Transition Metals in Proteins on a Microgram Scale
  • High Throughput Screening of Purified Proteins for Enzymatic Activity
  • Structural Characterization of Proteins
  • Strategies for Improving Crystallization Success Rates
  • Protein Crystallization in Restricted Geometry: Advancing Old Ideas for Modern Times in Structural Proteomics
  • Fluorescence Approaches to Growing Macromolecule Crystals
  • Efficient Macromolecular Crystallization Using Microfluidics and Randomized Design of Screening Reagents
  • Increasing Protein Crystallization Screening Success with Heterogeneous Nucleating Agents
  • High Throughput pH Optimization of Protein Crystallization
  • Automated Structure Solution with the PHENIX Suite
  • NMR Screening for Rapid Protein Characterization in Structural Proteomics
  • Microcoil NMR Spectroscopy: a Novel Tool for Biological High Throughput NMR Spectroscopy
  • Protein Structure Determination Using a Combination of Cross-linking, Mass Spectrometry, and Molecular Modeling
  • Structural Proteomics Initiatives Overviews
  • Structural Genomics of Minimal Organisms: Pipeline and Results
  • Structural Genomics of Pathogenic Protozoa: an Overview
  • High Throughput Crystallography at SGC Toronto: an Overview
  • The Structural Biology and Genomics Platform in Strasbourg: an Overview
  • Bacterial Structural Genomics Initiative: Overview of Methods and Technologies Applied to the Process of Structure Determination
  • High Throughput Protein Production and Crystallization at NYSGXRC
  • Overview of the Pipeline for Structural and Functional Characterization of Macrophage Proteins at the University of Queensland
  • Structural Genomics of the Bacterial Mobile Metagenome: an Overview.