Lipid Signaling Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Larijani, Banafshé. (Editor ), Woscholski, Rudiger. (Editor ), Rosser, Colin A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 462
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-60327-115-8
Tabla de Contenidos:
  • Lipid Systems and Techniques
  • Eicosanoids: Generation and Detection in Mammalian Cells
  • Lipidomic Analysis of Phospholipids and Related Structures by Liquid Chromatography-Mass Spectrometry
  • Measurement of Polyphosphoinositides in Cultured Mammalian Cells
  • Inositol Lipid-Dependent Functions in : Analysis of Phosphatidylinositol Phosphates
  • Methods for the Determination of the Mass of Nuclear PtdIns4, PtdIns5, and PtdIns(4,5)
  • Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea Urchin
  • Solution and Solid-State NMR of Lipids
  • Spatial Localization of PtdIns in Phase-Separated Giant Unilamellar Vesicles with a Fluorescent PLC-delta 1 PH Domain
  • Studying Cell-to-Cell Interactions: An Easy Method of Tethering Ligands on Artificial Membranes
  • Ceramide-Induced Transbilayer (Flip-Flop) Lipid Movement in Membranes
  • Effects of Sterols on the Development and Aging of
  • Proteomic Analysis of the Sterol-Mediated Signaling Pathway in
  • Lipid Metabolism and Recognition
  • Studying the Subcellular Localization and DNA-Binding Activity of FoxO Transcription Factors, Downstream Effectors of PI3K/Akt
  • Measurement of PTEN Activity by Imaging Phosphorylated Akt
  • Analysis of Phosphatidylinositol 3,4,5 Trisphosphate 5-Phosphatase Activity by and Assays
  • Measuring Phospholipase D Activity in Insulin-Secreting Pancreatic #x03B2;-Cells and Insulin-Responsive Muscle Cells and Adipocytes
  • Protein Kinase C as an Effector of Lipid-Derived Second Messengers
  • Detection of Myotubularin Phosphatases Activity on Phosphoinositides and
  • Quantification of Multiple Phosphatidylinositol 4-Kinase Isozyme Activities in Cell Extracts
  • Phospholipid-Interacting Proteins by Solution-State NMR Spectroscopy
  • Revealing Signaling in Single Cells by Single- and Two-Photon Fluorescence Lifetime Imaging Microscopy
  • Phosphoinositide (PI) 3-Kinase Assays
  • Measurement of Phosphatidylinositol and Phosphatidylcholine Binding and Transfer Activity of the Lipid Transport Protein PITP
  • Reconstitution of Activation of PLC#x03B5; by Ras and Rho GTPases
  • Assaying Endogenous Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K) Activities
  • Preparation of Membrane Rafts.