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02779nam a22003735i 4500 |
| 001 |
978-1-60327-977-2 |
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20191024171223.0 |
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cr nn 008mamaa |
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100301s2009 xxu| s |||| 0|eng d |
| 020 |
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|a 9781603279772
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| 024 |
7 |
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|a 10.1007/978-1-60327-977-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Zebrafish
|b Methods and Protocols /
|c edited by Graham J. Lieschke, Andrew C. Oates, Koichi Kawakami.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XI, 335 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 546
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| 505 |
0 |
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|a Mutagenesis and Mutants -- Highly Efficient ENU Mutagenesis in Zebrafish -- Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish -- Undertaking a Successful Gynogenetic Haploid Screen in Zebrafish -- Cryopreservation and In Vitro Fertilization at the Zebrafish International Resource Center -- Transgenesis -- Transient and Stable Transgenesis Using Tol2 Transposon Vectors -- Analysis of Genes and Genome by the Tol2-Mediated Gene and Enhancer Trap Methods -- Bacterial Artificial Chromosome Transgenesis for Zebrafish -- Simple and Efficient Transgenesis with Meganuclease Constructs in Zebrafish -- Tissue-Specific Manipulations -- Nitroreductase-Mediated Cell Ablation in Transgenic Zebrafish Embryos -- Focal Electroporation in Zebrafish Embryos and Larvae -- Tissue Micromanipulation in Zebrafish Embryos -- Analyzing Gene Expression -- Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments. Part I: Array Printing and Hybridization -- Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments: Data Acquisition and Analysis -- Validating microRNA Target Transcripts Using Zebrafish Assays -- Imaging -- Multiple Embryo Time-Lapse Imaging of Zebrafish Development -- Live Cell Imaging of Zebrafish Leukocytes -- Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy -- Morphological Analysis of the Zebrafish Digestive System -- In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy.
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| 650 |
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0 |
|a Wildlife.
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| 650 |
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0 |
|a Fish.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Animal models in research.
|
| 650 |
1 |
4 |
|a Fish & Wildlife Biology & Management.
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| 650 |
2 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Animal Models.
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| 700 |
1 |
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|a Lieschke, Graham J.
|e editor.
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| 700 |
1 |
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|a Oates, Andrew C.
|e editor.
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| 700 |
1 |
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|a Kawakami, Koichi.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
|
| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-60327-977-2
|