Methods in Endothelial Cell Biology
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
2004.
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| Edition: | 1st ed. 2004. |
| Series: | Springer Lab Manuals
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| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-3-642-18725-4 |
Table of Contents:
- I: Isolation and Culture of Endothelial Cells
- 1 Isolation, Purification, and Culture of Human Micro- and Macrovascular Endothelial Cells
- 2 Isolation of Mouse Cardiac Microvascular Endothelial Cells
- 3 Isolation, Culture, and Differentiation of Circulating Endothelial Precursor Cells
- 4 Embryonic Stem Cell-Derived Endothelial Cells
- 5 Detection, Isolation, and Culture Of Lymphatic Endothelial Cells
- 6 Three-Dimensional Spheroid Culture of Endothelial Cells
- 7 Immortalization of Endothelial Cells
- 8 Viral Gene Transfer Into Endothelial Cells
- II: Functional Assays in Vitro
- 9 Leukocyte Adhesion to Endothelium in Vitro Under Shear Stress
- 10 Application of Biomechanical Forces on Endothelial Cells
- 11 Endothelial Cell Permeability Assays in Culture
- 12 Three-Dimensional in Vitro Angiogenesis Assays
- 13 Rat Aortic Ring Assay of Angiogenesis
- 14 Endothelial Cell Chemotaxis and Chemokinesis Assays
- 15 FACS Analysis of Endothelial Cells
- 16 Application of the RNA Interference (RNAi) Technology to Angiogenesis Research
- III: Functional Assays in Vivo
- 17 Murine Bone Marrow Transplantation Models that Enable the Study of EPC Recruitment
- 18 Balloon Denudation of Blood Vessels
- 19 Peripheral Hind Limb Ischemia Models
- 20 In Vivo Matrigel Angiogenesis Assays
- 21 Analysis of Vascular Permeability in vivo
- 22 Validating the Manipulation of Endothelial Cell signaling in Vivo
- 23 Chick Chorioallantoic Membrane Assay: Growth Factor and Tumor-induced Angiogenesis and Lymphangiogenesis
- 24 Corneal Angiogenesis Assay
- IV: Detection of Endothelial Cells
- 25 Basic Blood Vessel Immunohistochemistry
- 26 Double-Staining Immunohistochemistry of Blood Vessels
- 27 In Situ Hybridization Analysis of Vascular Endothelium
- 28 High-Resolution in Situ Confocal Analysis of Endothelial Cells
- 29 Whole Mount Analysis of the Embryonic Vasculature
- 30 Methods for Visualizing Intact Blood Vessels
- 31 Intravital Multi-Fluorescence Microscopy
- 32 Corrosion Cast Analysis of Blood Vessels
- Suppliers
- Endothelial Cell Biology on the Internet.