Molecular Genetic Epidemiology A Laboratory Perspective /

Molecular Genetic Epidemiology A Laboratory Perspective /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Day, Ian N.M. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2002.
Edición:1st ed. 2002.
Colección:Principles and Practice,
Materias:
Cell biology.
Biochemistry.
Human genetics.
Molecular biology.
Cancer research.
Cell Biology.
Biochemistry, general.
Human Genetics.
Molecular Medicine.
Cancer Research.
Acceso en línea:https://doi.org/10.1007/978-3-642-56207-5
Tabla de Contenidos:
  • 1 Mapping Genes for Common Diseases: Statistical Planning, Power, Efficiency and Informatics
  • 1.1 Introduction
  • 1.2 Power and Sampling Considerations
  • 1.3 Models of Locus Action
  • 1.4 Maximum Likelihood Estimation
  • 1.5 Allelic Association
  • 1.6 The Malecot Model for Association
  • 1.7 Candidate Genes for Allelic Association
  • 1.8 Significance Levels
  • 1.9 Meta-Analysis
  • 1.10 Informatics
  • 1.11 Summary
  • References
  • 2 Human DNA Sampling and Banking
  • 2.1 Introduction
  • 2.2 A Brief History of DNA Research
  • 2.3 Goals and Needs of Human Population Genetics
  • 2.4 Selection of the Source Tissue
  • 2.5 A Fundamental Change in Sampling Methodology
  • 2.6 DNA Isolation and Purification
  • 2.7 Sample Storing
  • 2.8 Sample Banking
  • References
  • 3 Microsatellite Genotyping
  • 3.1 Introduction
  • 3.2 Microsatellite Markers
  • 3.3 The Genotyping Process - Experimental Considerations
  • 3.4 Data Analysis
  • 3.5 Data Management
  • 3.6 Hardware
  • 3.7 Future Developments
  • 3.8 Applications
  • References
  • 4 Minisatellite and Microsatellite DNA Fingerprinting
  • 4.1 Introduction
  • 4.2 Blood Grouping
  • 4.3 DNA Fingerprinting — The Discovery
  • 4.4 DNA Fingerprinting — The Applications
  • 4.5 An Explosion of DNA Fingerprinting Sequences
  • 4.6 Shortcomings of Multilocus Probes
  • 4.7 Single Locus Probes — DNA Profiling
  • 4.8 Europe and the USA Disagree
  • 4.9 Lessons from the Castro Case
  • 4.10 Impact of PCR
  • 4.11 Digital DNA Typing
  • 4.12 Short Tandem Repeat Profiling
  • 4.13 The UK National DNA Database
  • 4.14 STR Profiling Applications
  • 4.15 The Future
  • References
  • 5 Multiplex Polymerase Chain Reaction and Immobilized Probes: Application to Cardiovascular Disease
  • 5.1 Introduction
  • 5.2 Multiplex Polymerase Chain Reaction (PCR) Methodology
  • 5.3 Sequence-Specific Oligonucleotide Probe (SSOP) Methodology
  • 5.4 Example: Candidate Markers of CVD Risk
  • 5.5 Summary
  • References
  • 6 The Special Case of HLA Genes: Detection and Resolution of Multiple Polymorphic Sites in a Single Gene
  • 6.1 Introduction
  • 6.2 The HLA System
  • 6.3 Requirement for HLA Genotyping: Historical Perspectives
  • 6.4 PCR-based Approaches to HLA Genotyping
  • 6.5 A PCR-SSOP-Based Strategy for HLA Class II Typing Using DNA Derived from Archival Tissue Banks
  • 6.6 Concluding Remarks
  • References
  • 7 Microplate Array Diagonal Gel Electrophoresis (MADGE) Methodologies: The First Five Years
  • 7.1 Introduction
  • 7.2 Microplate Array Diagonal Gel Electrophoresis (MADGE)
  • 7.3 CpG-PCR
  • 7.4 Temporal Thermal Ramp MADGE Electrophoresis (Melt-MADGE)
  • 7.5 Software Applications (Applies to All MADGE Not Just Melt-MADGE)
  • 7.6 Future Developments
  • References
  • 8 The Use of Sequence Analysis for Homozygote and Heterozygote Base Variation Discovery
  • 8.1 Introduction
  • 8.2 Sequence Analysis: a Polymorphism Discovery Method
  • 8.3 Key Advancements in Sequencing Technologies: Chemistries, Hardware, and Software
  • 8.4 Heterozygote and Homozygote Polymorphism Base Calling
  • 8.5 Strategies for Sequencing-Based Polymorphism Detection
  • 8.6 Summary and Outlook: To Sequence or Not To Sequence  — This Is Not in Question
  • References.

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