Rapid Cycle Real-Time PCR Methods and Applications /
| Autor Corporativo: | |
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| Otros Autores: | , , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
2001.
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| Edición: | 1st ed. 2001. |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-3-642-59524-0 |
Tabla de Contenidos:
- Rapid Cycle Real-Time PCR: Methods and Applications
- I Methods
- Mutation Detection by Fluorescent Hybridization Probe Melting Curves
- Quantification on the LightCycler
- Selection of Hybridization Probes for Real-Time Quantification and Genetic Analysis
- Using the Nearest Neighbor Model for the Estimation of Matched and Mismatched Hybridization Probe Melting Points and Selection of Optimal Probes on the LightCycler
- Quantification of Human Papilloma Virus Type 16 Using Quantitative Competitive PCR on the LightCycler
- Use of TaqStart Antibody to Increase the Sensitivity of Herpesvirus Quantitative PCR on the LightCycler
- II Genotyping of Human Germline Variations
- High-Speed Detection of ?1-Antitrypsin Deficiency Alleles Pi*S and Pi*Z on the LightCycler
- High-Speed Methylenetetrahydrofolate Reductase C ? T 677 Mutation Detection on the LightCycler
- Dual Color Detection of Splice Variants of the c-erbA ? (Thyroid Hormone Receptor ?) Gene
- Detection of Three Major Polymorphisms in the N-Acetyltransferase 2 Gene by Melting Peak Analysis Using Fluorogenic Hybridization Probes
- Genotyping of Cytochrome P450 2D6*4 Mutation with Fluorescent Hybridization Probes Using LightCycler
- Fluorescent Hybridization Probe Detection of the F508de1 Cystic Fibrosis Allele on the LightCycler
- Genotyping ?-globin Mutations (Hb S, Hb C, Hb E) by Multiplexing Probe Color and Melting Temperature
- Simultaneous Detection of C282Y and H63D Hemochromatosis Mutations Using LCRed 640 and LCRed 705 Labeled Hybridization Probes
- Genotyping of Angiotensin-Converting Enzyme and Angiotensinogen Polymorphisms with the LightCycler System
- Genotyping of the Most Common Thiopurine Methyltransferase Mutations with the LightCycler
- Detection of the Mitochondria) DNA Mutation MELAS3243 Using Hybridization Probes
- III Acquired Genetic Alterations in Human Diseases
- Detection of p53 Allele Deletions in Human Cancer by Quantification of Genomic Copy Number
- Monitoring of Residual Disease in Patients with Chronic Myelogenous Leukemia Using Specific Fluorescent Hybridization Probes for Real-Time Quantitative RT-PCR
- Development of Quantitative RT-PCR for the Expression of Wilms’ Tumor WT1 Suppressor Gene in Leukemia on the LightCycler
- Real-Time Detection of Minimal Residual Disease by Amplifying Immunoglobulin Genes in Acute Lymphoblastic Leukemia on the LightCycler
- HERZ/neu Gene Amplification Quantified by PCR and Melting Peak Analysis Using a Single Base Alteration Competitor as an Internal Standard
- Quantification of Residual Tumor Cells in Monoclonal B-cell Lymphoma
- Development of PCR-Based Assays for the Detection of Chromosomal Translocations Using SYBR Green I
- Relative Quantification of the HER2/neu Oncogene Using SYBR Green I
- IV Receptors and Mediators
- Development of Quantitative RT-PCR Tests for the Expression of Cytokine Genes on the LightCycler
- Quantitative RT-PCR for the Detection of T Cell Receptor Transcripts in T Lymphocytes Populations Using LightCycler Technology
- Rapid, Homogeneous Genotyping of Human Platelet Antigen 1 by Fluorescence Resonance Energy Transfer and Probe Melting Curves
- Development and Validation of an Externally Standardised Quantitative Insulin-like Growth Factor-1 RT-PCR Using LightCycler SYBR Green I Technology
- An Application of Melting Curve Analysis to Large-Scale Genetic Analysis in Atherosclerotic Disease: Two Linked Polymorphisms of Glycoprotein la Gene and Myocardial Infarction in Japanese
- V Infectious Organisms
- Genotype-Specific Analysis of Hepatitis B Virus DNA on the LightCycler
- Rapid and Specific Detection of Bordetella pertussis in Clinical Specimens by LightCycler PCR
- Rapid and Specific Detection of Helicobacter pylori by LightCycler PCR
- Qualitative Detection of Herpes Simplex Virus DNA on the LightCycler
- Quantitative Detection of Cryptosporidium parvum after In Vitro Excystation by LightCycler PCR
- Quantitative Analysis of CMV in Infected Mice on the LightCycler System
- Detection and Differentiation of Equine Herpes Virus Type 1 and Type 4 on the LightCycler
- Rapid and Quantitative Detection of Toxoplasma gondii by PCR — A LightCycler Application in Prenatal Diagnosis
- Development of Quantitative PCR Tests for the Detection of the Orthopox Virus Adsorption Protein Gene (ORF D8L) on the LightCycler
- VI Plant Gene Products and Miscellaneous
- Quantification of Genetically Modified Soybeans in Food with the LightCycler System
- Real-Time PCR Monitoring of Estuarine Water Samples for Pfiesteria piscicida: A Dinoflagellate Associated with Fish Kills and Human Illness
- Quantification of Retrotransposon XIR-2.5 Copy Number in Genomes of Poeciliidae Species.