Differential-Display Reverse Transcription-PCR (DDRT-PCR)

Differential-Display Reverse Transcription-PCR (DDRT-PCR)

Detalles Bibliográficos
Autores principales: Colonna-Romano, Sergio. (Autor), Leone, Antonella. (Autor), Maresca, Bruno. (Autor)
Autor Corporativo: SpringerLink (Online service)
Formato: eBook
Lenguaje:English
Publicado: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1998.
Edición:1st ed. 1998.
Colección:Springer Lab Manuals
Materias:
Cell biology.
Biochemistry.
Cell Biology.
Biochemistry, general.
Acceso en línea:https://doi.org/10.1007/978-3-642-80454-0
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024 7 |a 10.1007/978-3-642-80454-0  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
100 1 |a Colonna-Romano, Sergio.  |e author. 
245 1 0 |a Differential-Display Reverse Transcription-PCR (DDRT-PCR)  |c by Sergio Colonna-Romano, Antonella Leone, Bruno Maresca. 
250 |a 1st ed. 1998. 
260 # # |a Berlin, Heidelberg :  |b Springer Berlin Heidelberg :  |b Imprint: Springer,  |c 1998. 
300 |a X, 124 p. 74 illus.  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Springer Lab Manuals 
505 0 |a Overview -- 1 Differential Display of mRNAs: Principles and General Applications -- 2 DDRT-PCR: A Brief Description -- 3 Summary -- 1 Preparation of Total RNA -- 1.1 Extraction from Yeast by Phenol -- 1.2 Extraction by Guanidine Thiocyanate, Plant Tissue -- 1.3 Extraction by Guanidine Thiocyanate, Animal Cells -- 1.4 Extraction from Fish Liver -- 1.5 RNA Quantification -- 1.6 DNase Treatment -- 1.7 Verification of Integrity -- 2 Differential Display -- 2.1 Reverse Transcription of Total RNA -- 2.2 PCR Amplification -- 3 Size Separation of cDNA Fragments -- 3.1 Gel Electrophoresis -- 3.2 End Labeling of DNA Markers -- 4 Isolation of Differentially Expressed cDNA Fragments -- 4.1 Alignment of a Differential-Display Gel Using a Film Image -- 4.2 Elution of cDNA Fragments from Gel -- 5 Reamplification of Eluted cDNA -- 6 Cloning of Amplified cDNA Fragments -- 6.1 Purification of cDNA Fragments from Agarose -- 6.2 Ligation of cDNA into Vectors -- 6.3 Preparation of CaCl2 Competent Cells for Transformation -- 6.4 Preparation of Electrocompetent Cells -- 6.5 Transformation of CaCl2 Competent Cells -- 6.6 Transformation of Competent Cells by Electroporation -- 7 Checking Subcloned Fragments -- 8 Confirmation of Differential Expression of Cloned cDNA Fragments -- 8.1 Slot and Northern Blots -- 8.2 DNA Labeling and Hybridization -- 8.3 Ribonuclease Protection Assay -- 8.4 Nuclear Run-On Assay -- 9 Northern Blot Affinity Capturing of cDNA -- 9.1 PCR Labeling of Differentially Expressed cDNA -- 9.2 Northern Blot Affinity Capturing -- 10 Sequencing of Differentially Expressed cDNA Fragments -- 10.1 Plasmid DNA Preparation -- 10.2 Sequencing Reaction with Sequenase Version 2.0 T7 DNA Polymerase -- 10.3 Sequencing Reaction with Thermo-Sequenase Cycle-Sequencing Kit -- Materials, Equipment, Reagents, and Suppliers -- References. 
650 0 |a Cell biology. 
650 0 |a Biochemistry. 
650 1 4 |a Cell Biology. 
650 2 4 |a Biochemistry, general. 
700 1 |a Leone, Antonella.  |e author. 
700 1 |a Maresca, Bruno.  |e author. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-3-642-80454-0 

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