THE BIOSYNTHESIS OF ROSMARINIC ACID IN SUSPENSION CULTURES OF COLEUS BLUMEI /

Suspension cultures of Coleus blumei accumulate very high amounts of rosmarinic acid, an ester of caffeic acid and 3,4-dihydroxyphenyllactate, in medium with elevated sucrose concentrations. Since the synthesis of this high level of rosmarinic acid occurs in only five days of the culture period, the...

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Lenguaje:Spanish
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040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 4 |a THE BIOSYNTHESIS OF ROSMARINIC ACID IN SUSPENSION CULTURES OF COLEUS BLUMEI /  |c MAIKE PETERSEN ... [ET AL.]. 
520 |a Suspension cultures of Coleus blumei accumulate very high amounts of rosmarinic acid, an ester of caffeic acid and 3,4-dihydroxyphenyllactate, in medium with elevated sucrose concentrations. Since the synthesis of this high level of rosmarinic acid occurs in only five days of the culture period, the activities of the enzymes involved in the biosynthesis are very high. Therefore all the enzymes necessary for the formation of rosmarinic acid from the precursors phenylalanine and tyrosine could he isolated from cell cultures of Coleus blumei: phenylalanine ammonia-lyase, cinnamic acid 4-hydroxylase, hydroxycinnamoyl:CoA ligase, tyrosine aminotransferase, hydroxyphenylpyruvate reductase, rosmarinic acid synthase and two microsomal 3- and 3`-hydroxylases. The main characteristics of these enzymes of the proposed biosynthetic pathway of rosmarinic acid will he describes. 
650 4 |a COLEUS BLUMEI 
650 4 |a TIROSINA 
650 4 |a PLANTAS ORNAMENTALES 
650 4 |a METABOLISMO 
650 4 |a LABIATAE 
650 4 |a BIOSINTESIS 
650 4 |a BIOTECNOLOGIA 
650 4 |a FENALALANINA 
650 4 |a AMINOACIDOS ESENCIALES 
655 4 |a Artículos de revista 
773 1 |t PLANT CELL, TISSUE AND ORGAN CULTURE  |g Vol. 38, núm.2-3 (septiembre, 1994), páginas 171-179 
905 |a BSC_ANA