Autophagy : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Ktistakis, Nicholas. (Editor ), Florey, Oliver. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1880
Materias:
Tabla de Contenidos:
  • Recombinant Expression, Purification and Assembly of p62 Filaments
  • Structural Studies of Autophagy-related Proteins
  • Structural Studies of Mammalian Autophagy Lipidation Complex
  • Structural Studies of Selective Autophagy in Yeast
  • Biophysical Studies of LC3 Family Proteins
  • Reconstituting Autophagy Initiation from Purified Components
  • Cell-free Reconstitution of Autophagic Membrane Formation
  • Use of Peptide Arrays for Identification and Characterization of LIR Motifs
  • Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System
  • Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature
  • Studies of Receptor-Atg8 Interactions during Selective Autophagy
  • Correlative Light and Electron Miscopy of Autophagosomes
  • Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells
  • 3-color Simultaneous Live Imaging of Autophagy-related Structures
  • Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy
  • Methods for Imaging Autophagosome Dynamics in Primary Neurons
  • Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinson’s Disease Research
  • Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo
  • Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells
  • Measurement of Bulk Autophagy by a Cargo Sequestration Assay
  • Methods to Detect Loss of Lysosomal Membrane Integrity
  • ­­Monitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress
  • Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches
  • Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen
  • Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening
  • In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells
  • In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells
  • Methods for the Study of Entotic Cell Death
  • MHC Class I Internalization via Autophagy Proteins
  • Analysis of Autophagy for Liver Pathogenesis
  • Autophagy in 3D In Vitro and Ex Vivo Cancer Models
  • Autophagy in Platelets
  • Methods to Image Macroautophagy in the Brain In Vivo
  • Measuring Nonselective and Selective Autophagy in the Liver
  • Measuring Autophagy in Pancreatitis
  • Characterization of the “autophagic flux” in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy
  • Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing
  • Investigating Non-selective Autophagy in Drosophila
  • Imaging the Dynamics of Mitophagy in Live Cells
  • Triggering Mitophagy with Photosensitizers
  • Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QC
  • Assays to Monitor Mitophagy in Drosophila
  • Mitophagy Dynamics in Caenorhabditis elegans
  • Methods for Studying Mitophagy in Yeast
  • Measuring Anti-bacterial Autophagy
  • Quantitative Phosphoproteomics of Selective Autophagy Receptors
  • Analysis of Chaperone-mediated Autophagy
  • Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations.