Autophagy : Methods and Protocols /
Autor Corporativo: | |
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Otros Autores: | , |
Formato: | eBook |
Lenguaje: | English |
Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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Edición: | 1st ed. 2019. |
Colección: | Methods in Molecular Biology,
1880 |
Materias: |
Tabla de Contenidos:
- Recombinant Expression, Purification and Assembly of p62 Filaments
- Structural Studies of Autophagy-related Proteins
- Structural Studies of Mammalian Autophagy Lipidation Complex
- Structural Studies of Selective Autophagy in Yeast
- Biophysical Studies of LC3 Family Proteins
- Reconstituting Autophagy Initiation from Purified Components
- Cell-free Reconstitution of Autophagic Membrane Formation
- Use of Peptide Arrays for Identification and Characterization of LIR Motifs
- Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System
- Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature
- Studies of Receptor-Atg8 Interactions during Selective Autophagy
- Correlative Light and Electron Miscopy of Autophagosomes
- Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells
- 3-color Simultaneous Live Imaging of Autophagy-related Structures
- Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy
- Methods for Imaging Autophagosome Dynamics in Primary Neurons
- Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinson’s Disease Research
- Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo
- Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells
- Measurement of Bulk Autophagy by a Cargo Sequestration Assay
- Methods to Detect Loss of Lysosomal Membrane Integrity
- Monitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress
- Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches
- Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen
- Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening
- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells
- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells
- Methods for the Study of Entotic Cell Death
- MHC Class I Internalization via Autophagy Proteins
- Analysis of Autophagy for Liver Pathogenesis
- Autophagy in 3D In Vitro and Ex Vivo Cancer Models
- Autophagy in Platelets
- Methods to Image Macroautophagy in the Brain In Vivo
- Measuring Nonselective and Selective Autophagy in the Liver
- Measuring Autophagy in Pancreatitis
- Characterization of the “autophagic flux” in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy
- Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing
- Investigating Non-selective Autophagy in Drosophila
- Imaging the Dynamics of Mitophagy in Live Cells
- Triggering Mitophagy with Photosensitizers
- Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QC
- Assays to Monitor Mitophagy in Drosophila
- Mitophagy Dynamics in Caenorhabditis elegans
- Methods for Studying Mitophagy in Yeast
- Measuring Anti-bacterial Autophagy
- Quantitative Phosphoproteomics of Selective Autophagy Receptors
- Analysis of Chaperone-mediated Autophagy
- Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations.