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03006nam a22003135i 4500 |
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20200922153935.0 |
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cr nn 008mamaa |
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190828s2020 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493996988
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| 024 |
7 |
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|a 10.1007/978-1-4939-9698-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a ADAMTS Proteases :
|b Methods and Protocols /
|c edited by Suneel S. Apte.
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| 250 |
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|a 1st ed. 2020.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2020.
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| 300 |
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|a X, 299 p. 59 illus., 47 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 2043
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| 505 |
0 |
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|a ADAMTS Proteins: Concepts, Challenges, and Prospects -- Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing Technology -- Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins using Cell-Based Assays -- Challenges and Solutions for Purification of ADAMTS Proteases: An Overview -- Purification of Native or Recombinant ADAMTS2 and Procollagen I Cleavage Assay -- ADAMTS7: Recombinant Protein Expression and Purification -- Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted Mutants -- Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins -- Assay for Reelin-Cleaving Activity of ADAMTS and Detection of Reelin and Its Fragments in the Brain -- Chemical Modification of Proteoglycanases with Biotin -- Analysis of Aggrecanase Activity using Neoepitope Antibodies -- Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4 -- Purification of Recombinant ADAMTSL2 -- Expression Analysis by RNAscope™ In Situ Hybridization -- Analysis of ADAMTS Effects on Cell Adhesion and Migration -- Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils -- Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton -- Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins -- Quantification of Extracellular Matrix Fiber Systems Related to ADAMTS Proteins -- Visualization of Perineuronal Nets in Central Nervous System Tissue Sections -- Visualization and Quantification of Pericellular Matrix -- Quantitative Mass Spectrometry-Based Secretome Analysis as a Tool to Investigate Metalloprotease and TIMP Activity -- Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates -- Mapping the N-Terminome in Tissue Biopsies by PCT-TAILS. .
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| 650 |
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|a Proteins .
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| 650 |
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|a Proteomics.
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| 650 |
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4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Proteomics.
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| 700 |
1 |
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|a Apte, Suneel S.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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