ADAMTS Proteases : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2020.
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| Edición: | 1st ed. 2020. |
| Colección: | Methods in Molecular Biology,
2043 |
| Materias: |
Tabla de Contenidos:
- ADAMTS Proteins: Concepts, Challenges, and Prospects
- Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing Technology
- Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins using Cell-Based Assays
- Challenges and Solutions for Purification of ADAMTS Proteases: An Overview
- Purification of Native or Recombinant ADAMTS2 and Procollagen I Cleavage Assay
- ADAMTS7: Recombinant Protein Expression and Purification
- Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted Mutants
- Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins
- Assay for Reelin-Cleaving Activity of ADAMTS and Detection of Reelin and Its Fragments in the Brain
- Chemical Modification of Proteoglycanases with Biotin
- Analysis of Aggrecanase Activity using Neoepitope Antibodies
- Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4
- Purification of Recombinant ADAMTSL2
- Expression Analysis by RNAscope™ In Situ Hybridization
- Analysis of ADAMTS Effects on Cell Adhesion and Migration
- Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils
- Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton
- Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins
- Quantification of Extracellular Matrix Fiber Systems Related to ADAMTS Proteins
- Visualization of Perineuronal Nets in Central Nervous System Tissue Sections
- Visualization and Quantification of Pericellular Matrix
- Quantitative Mass Spectrometry-Based Secretome Analysis as a Tool to Investigate Metalloprotease and TIMP Activity
- Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates
- Mapping the N-Terminome in Tissue Biopsies by PCT-TAILS. .