Amyloid Proteins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Sigurdsson, Einar M. (Editor ), Calero, Miguel. (Editor ), Gasset, María. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:3rd ed. 2018.
Colección:Methods in Molecular Biology, 1779
Materias:
Tabla de Contenidos:
  • Preparation of Pure Populations of Amyloid-β Protein Oligomers of Defined Size
  • Preparation of Well-Defined and Stable β-Barrel Pore-Forming Amyloid-β42 Oligomers
  • Unveiling Brain Aβ Heterogeneity through Targeted Proteomic Analysis
  • Preparation of α-Synuclein Amyloid Assemblies for Toxicity Experiments
  • Generation and Characterization of Stable α-Synuclein Oligomers
  • In Vitro Analysis of α-Synuclein Amyloid Formation and Cross-Reactivity
  • Preparation of Tau Oligomers after Protein Extraction from Bacteria and Brain Cortices
  • Purification and Characterization of Low-n Tau Oligomers
  • Preparation and Characterization of Tau Oligomer Strains
  • Purification and Fibrillation of Recombinant Human Amyloid-β, Prion Protein and Tau under Native Conditions
  • Preparation of Amyloidogenic Aggregates from EF-Hand β –parvalbumin and S100 Proteins
  • Kinetic Analysis of Amyloid Formation
  • Mapping Amyloid Regions in Gad m 1 with Peptide Arrays
  • Non-Invasive Structural Analysis of Intermediate Species during Fibrillation: An Application of Small-Angle X-Ray Scattering
  • Analysis of Covalent Modifications of Amyloidogenic Proteins using Two-Dimensional Electrophoresis: Prion Protein and Its Sialylation
  • Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using The Real-Time Quaking-Induced-Conversion
  • Bacterial Amyloids
  • Addressing Intracellular Amyloidosis in Bacteria with RepA-WH1, a Prion-Like Protein
  • Study of Amyloids Using Yeast
  • Neurotoxic Ca2+ Signaling Induced by Amyloid-β Oligomers in Aged Hippocampal Neurons In Vitro
  • Preparation and Culturing of Human Primary Vascular Cells
  • Live Imaging of Pathological Tau Protein and Tau Antibodies in a Human Neuron-Like Cellular Model
  • Effects of Amyloid-β Peptide on the Biology of Human Neural Stem Cells
  • Development of Mouse Monoclonal Antibodies against Human Amyloid Fibril Proteins for Diagnostic and Research Purposes
  • Identification and Characterization of Amyloid-β Accumulation in Synaptic Mitochondria
  • Biochemical Properties of Pathology-Related Tau Species in Tauopathy Brains: An Extraction Protocol for Tau Oligomers and Aggregates
  • Tau Assembly into Filaments
  • Quantitative Metabolomics in Alzheimer´s Disease: Technical Considerations for Improved Reproducibility
  • Detecting Circulating MicroRNAs as Biomarkers in Alzheimer’s Disease
  • Luminescent Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues
  • Characterization of Amyloid-β Plaques and Autofluorescent Lipofuscin Aggregates in Alzheimer´s Disease Brain: A Confocal Microscopy Approach
  • In Vivo Imaging of Tauopathy in Mice
  • In Vivo Evaluation of Neuronal Transport in Murine Models of Neurodegeneration using Manganese-Enhanced MRI.