Cell-Based Assays for High-Throughput Screening : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Clemons, Paul A. (Editor ), Tolliday, Nicola J. (Editor ), Wagner, Bridget K. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 486
Materias:
Tabla de Contenidos:
  • Introduction: Cell-Based Assays for High-Throughput Screening
  • High-Throughput Screening of Model Bacteria
  • Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells
  • Large-Scale Small-Molecule Screen Using Zebrafish Embryos
  • Whole-Animal High-Throughput Screens: The C. elegans Model
  • Whole-Organism Screening: Plants
  • Fluorescence-Based Assays
  • Reporter Gene Assays
  • Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-Restoration Assay
  • Assay for Isolation of Inhibitors of Her2-Kinase Expression
  • High-Content Screening: Flow Cytometry Analysis
  • High-Throughput Real-Time PCR for Detection of Gene-Expression Levels
  • Interpretation of Uniform-Well Readouts
  • Extracting Rich Information from Images.