Bacterial Polysaccharides : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
1954 |
| Materias: |
Tabla de Contenidos:
- 1. Customisable cloning of whole polysaccharide gene clusters by yeast homologous recombination
- 2. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-negative Bacteria
- 3. Purification of Capsular Polysaccharide Complex from Gram Negative Bacteria
- 4. Simple protocol to purify cell wall polysaccharide from Gram+ bacteria and assess its structural integrity
- 5. Extraction and purification of wall bound polymers of Gram-positive bacteria
- 6. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan and Lipoarabinomannan
- 7. Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry
- 8. Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry
- 9. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for studies in cytokine production
- 10. O-Acetylation of Bacterial Cell Wall Polysaccharides
- 11. Designing glycosyltransferase expression constructs for improved purification, protein yield and crystallization
- 12. Utilization of fluorescently-tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, KpsC, from Escherichia coli
- 13. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O-Antigenic Polysaccharides in Enteric Bacteria
- 14. Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides
- 15. Enzymatic synthesis of the repeating unit oligosaccharides of Escherichia coli O104
- 16. Sulfhydryl labelling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis
- 17. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides
- 18. Glycosyltransferase Activity Assays Using Colorimetric Methods
- 19. In vitro characterization of a multidomain glycosyltransferase using fluorescently-tagged synthetic acceptors
- 20. Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa
- 21. Assaying fucosidase activity
- 22. In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis
- 23. High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus.