Ancient DNA : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Shapiro, Beth. (Editor ), Barlow, Axel. (Editor ), Heintzman, Peter D. (Editor ), Hofreiter, Michael. (Editor ), Paijmans, Johanna L. A. (Editor ), Soares, André E. R. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:2nd ed. 2019.
Colección:Methods in Molecular Biology, 1963
Materias:
Tabla de Contenidos:
  • Setting Up an Ancient DNA Laboratory
  • Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate
  • Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step
  • Extraction of Highly Degraded DNA from Ancient Bones and Teeth
  • Sampling and Extraction of Ancient DNA from Sediments
  • Extraction of Ancient DNA from Plant Remains
  • DNA Extraction from Keratin and Chitin
  • Double-Stranded Library Preparation for Ancient and Other Degraded Samples
  • A Method for Single-Stranded Ancient DNA Library Preparation
  • Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms
  • Whole-Genome Capture of Ancient DNA Using Homemade Baits
  • Generating RNA Baits for Capture-Based Enrichment
  • Hybridization Capture of Ancient DNA Using RNA Baits
  • Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA
  • Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis
  • Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA
  • Authentication and Assessment of Contamination in Ancient DNA
  • Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence.