Beta-Arrestins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Scott, Mark G. H. (Editor ), Laporte, Stéphane A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1957
Materias:
Tabla de Contenidos:
  • A Brief History of the b-Arrestins
  • b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling
  • Methods to Monitor the Trafficking of β-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET
  • Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle
  • Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer
  • Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET
  • Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin
  • Methods to Investigate Arrestins in Complex with Phosphodiesterases
  • Methods to Characterize Protein Interactions with β-arrestin in cellulo
  • Methods to Investigate the β-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling
  • Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing
  • Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners
  • Workflow Description to Dynamically Model β-Arrestin Signaling Networks
  • Proteomic Analysis of the β-Arrestin Interactome
  • Quantitating Ligand Bias using the Competitive Model of Ligand Activity
  • Methods to Investigate the Nucleocytoplasmic Shuttling Properties of b-Arrestins
  • Monitoring β-Arrestin2 Targeting to the Centrosome, Basal Body and Primary Cilium by Fluorescence Microscopy
  • A Mass Spectrometry-Based Structural Assay for Activation-Dependent Conformational Changes in β-Arrestins
  • Probing Arrestin Function using Intramolecular FlAsH-BRET Biosensors
  • Methods to Study the Roles of b-Arrestins in Meningococcal Signaling
  • Methods to Investigate the Roles for β-Arrestin2 in Allergic Inflammatory Airway Disease
  • Methods to Study Roles of β-Arrestins in the Regulation of Pancreatic β-Cell Function
  • Methods to Investigate β-Arrestin Function in Metabolic Regulation
  • Methods to Investigate the Role of b-Arrestin Signaling in Parkinson's Ddisease
  • Methods to Investigate β-Arrestin-1/β-Catenin Signaling in Ovarian Cancer Cells.