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03083nam a22003015i 4500 |
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20200922085423.0 |
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cr nn 008mamaa |
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180226s2018 xxu| s |||| 0|eng d |
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|a 9781493976348
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| 024 |
7 |
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|a 10.1007/978-1-4939-7634-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Bacterial Regulatory RNA :
|b Methods and Protocols /
|c edited by Véronique Arluison, Claudio Valverde.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XII, 395 p. 84 illus., 62 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1737
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| 505 |
0 |
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|a Workflow for a Computational Analysis of an sRNA Candidate in Bacteria -- Molecular Phylogenomics for the Identification and Characterization of trans-encoded Small Non-coding RNA Families -- Bioinformatic Approach for Prediction of CsrA/RsmA-regulating small RNAs in Bacteria -- Host-pathogen Transcriptomics by Dual RNA-seq -- Identification of New Bacterial Small RNA Targets using MS2 Affinity Purification Coupled to RNA Sequencing -- Assessment of External Guide Sequences’ (EGS) Efficiency as Inducers of RNase P-mediated Cleavage of mRNA Target Molecules -- Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-dependent Termination of Transcription in vitro -- A Method to Map Changes in Bacterial Surface Composition Induced by Regulatory RNAs -- Fluorescence-based Methods for Characterizing RNA interactions in vivo -- Mutational Analysis of sRNA-mRNA Base Pairing by Electrophoretic Mobility Shift Assay -- An Integrated Cell-free Assay to Study Translation Regulation by Small Bacterial Non-coding RNAs -- Quantitative Super-resolution Imaging of Small RNAs in Bacterial Cells -- Extraction and Analysis of RNA Isolated from Pure Bacteria—Derived outer Membrane Vesicles -- Absolute Regulatory Small Noncoding RNA Concentration and Decay Rates Measurements in Escherichia coli -- High-resolution, High-throughput Analysis of Hfq Binding Sites using UV-crosslinking and Analysis of cDNA (CRAC) -- Producing Hfq/Sm Proteins and sRNAs for Structural and Biophysical Studies of Ribonucleoprotein Assembly -- Single Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing -- Techniques to Analyse sRNA-protein Cofactor Self-assembly in vitro -- Sequence-specific Affinity Chromatography of RNA-binding Proteins from Bacterial Cells -- Identification of Small RNA-protein Partners in Plant Symbiotic Bacteria -- A Modular Genetic System for High-throughput Profiling and Engineering of Multi-target Small RNAs. .
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| 650 |
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|a Human genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Arluison, Véronique.
|e editor.
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| 700 |
1 |
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|a Valverde, Claudio.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|