CD95 : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edition: | 1st ed. 2017. |
| Series: | Methods in Molecular Biology,
1557 |
| Subjects: |
Table of Contents:
- Production of the Non-apoptotic Metalloprotease-cleaved CD95L and its Cytotoxic Recombinant Counterpart Designed Ig-CD95L
- CD95 Stimulation with CD95L and DISC Analysis
- Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8
- In vitro Evaluation of the Apoptosis Function in Human Activated T cells
- Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition
- Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization
- Generation and Application of Bioluminescent CD95 Ligand Fusion Proteins
- CD95-mediated Calcium Signaling
- CD95-mediated Proton Regulation
- Study of the CD95-mediated Non-apoptotic Signaling Pathway - PI3K
- Organelle Separation and Cell Signaling
- Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L
- Isolation of Lipid Rafts through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Receptor Localization in Raft Fractions
- Quantifying CD95/cl-CD95L Implications in Cell Mechanics And Membrane Tension by Atomic Force Microscopy Based Force Measurements
- Sketching of CD95 Oligomers by In Silico Investigations
- Site-specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventional and Phospho-Protein Affinity SDS-PAGE
- Detection of S-acylated CD95 by Acyl-biotin Exchange
- Exploration of Fas S-nitrosylation by the Biotin Switch Assay
- Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L
- Liquid Chromatography-High Resolution Mass Spectrometry Method to Study Sphingolipid Metabolism Changes in Response to CD95L
- CD95 and the MRL-lpr Mouse Model.