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Chromatin Remodeling : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Morse, Randall H. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edition:1st ed. 2012.
Series:Methods in Molecular Biology, 833
Subjects:
Table of Contents:
  • Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library
  • Measuring Dynamic Changes in Histone Modifications and Nucleosome Density during Activated Transcription in Budding Yeast
  • Monitoring the Effects of Chromatin Remodelers on Long-Range Interactions In Vivo
  • Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease
  • Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay (NuSA)
  • Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility
  • Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination
  • An Efficient Purification System for Native Minichromosome from S. cerevisiae
  • Simultaneous, Single-Molecule Detection of Endogenous C-5 DNA Methylation and Chromatin Accessibility Using MAPit
  • Analysis of Stable and Transient Protein-Protein Interactions
  • Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Recovery after Photobleaching (FRAP)
  • Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Correlation Spectroscopy (FCS) and Temporal Image Correlation Spectroscopy (TICS)
  • Analysis of Chromatin Structure in Plant Cells
  • Analysis of Histones and Histone Variants in Plants
  • Reconstitution of Modified Chromatin Templates for In Vitro Functional Assays
  • A Defined In Vitro System to Study ATP-Dependent Remodeling of Short Chromatin Fibers
  • In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning on Yeast DNA
  • Activator-Dependent Acetylation of Chromatin Model Systems
  • Mapping Assembly-Favored and Remodeled Nucleosome Positions on Polynucleosomal Templates
  • Analysis of Changes in Nucleosome Conformation Using Fluorescence Resonance Energy Transfer (FRET)
  • Preparation of Nucleosomes Containing a Specific H2A-H2A Crosslink Forming a DNA-Constraining Loop Structure
  • Sulfyhydryl Reactive Site-Directed Crosslinking as a Method for Probing the Tetrameric Structure of Histones H3 and H4
  • Genomic Approaches for Determining Nucleosome Occupancy in Yeast
  • Genome-Wide Approaches to Determining Nucleosome Occupancy in Metazoans Using MNase-Seq
  • Salt Fractionation of Nucleosomes for Genome-Wide Profiling
  • Quantitative Analysis of Genome-Wide Chromatin Remodeling
  • Computational Analysis of Nucleosome Positioning.