Cell Cycle Checkpoint Control Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Lieberman, Howard B. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edition:1st ed. 2004.
Series:Methods in Molecular Biology, 241
Subjects:
Table of Contents:
  • Induction and Detection of Changes in cell Cycle Progression
  • Methods to Induce Cell Cycle Checkpoints
  • Methods for Synchronizing Mammalian Cells
  • Enrichment of Cells in Different Phases of the Cell Cycle by Centrifugal Elutriation
  • Analysis of the Mammalian Cell Cycle by Flow Cytometry
  • Methods for Detecting Cells in S Phase
  • Yeast Cell Synchronization
  • Analysis of the Budding Yeast Saccharomyces cerevisiae Cell Cycle by Morphological Criteria and Flow Cytometry
  • Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle
  • Analysis of Genes Involved in Checkpoint Control
  • Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes
  • Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
  • Using the Yeast Genome-Wide Gene-Deletion Collection for Systematic Genetic Screens
  • Gene Targeting in Cultured Human Cells
  • Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene
  • In Vitro Mutagenesis to Define Functional Domains
  • Use of Gene Overexpression to Assess Function in Cell Cycle Control
  • Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression
  • Analysis of Proteins Involved in Checkpoint Control
  • Cataloging Proteins in Cell Cycle Control
  • Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control
  • Purification and Identification of Protein Complexes That Control the Cell Cycle
  • Xenopus Cell-Free Extracts to Study DNA Damage Checkpoints
  • Protein-Protein Interactions
  • Detection of Kinase and Phosphatase Activities
  • Monitoring Changes in the Subcellular Location of Proteins in S. cerevisiae
  • Chromosomes and the Cell Cycle
  • Chromosomal Changes and Cell Cycle Checkpoints in Mammalian Cells
  • Detecting the Influence of Cell Cycle Regulatory Proteins on Human Telomeres
  • Monitoring Spindle Assembly and Disassembly in Yeast by Indirect Immunofluorescence.