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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592597529
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| 024 |
7 |
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|a 10.1385/1592597521
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Bacterial Artificial Chromosomes :
|b Volume 1: Library Construction, Physical Mapping, and Sequencing /
|c edited by Shaying Zhao, Marvin Stodolsky.
|
| 250 |
|
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|a 1st ed. 2004.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
|
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|a XX, 360 p. 71 illus. :
|b online resource.
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
|
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
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|a Methods in Molecular Biology,
|v 255
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| 505 |
0 |
|
|a BAC Library Construction -- Construction of Small Genome BAC Library for Functional and Genomic Applications -- Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging -- Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions -- Purification of BAC DNA -- Hybridization-Based Selection of BAC Clones -- Applications of Interspersed Repeat Sequence Polymerase Chain Reaction -- BAC Mapping Using Fluorescence In Situ Hybridization -- High-Throughput BAC Fingerprinting -- BAC End Sequencing -- Radiation Hybrid Mapping With BAC Ends -- Shotgun Library Construction for DNA Sequencing -- Rolling Circle Amplification for Sequencing Templates -- Transposon-Mediated Sequencing -- Pyrosequencing -- Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions -- Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions -- Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers -- BAC Finishing Strategies -- Using the TIGR Assembler in Shotgun Sequencing Projects -- Finishing “Working Draft” BAC Projects by Directed Sequencing With ThermoFidelase and Fimers -- Optimized Multiplex Polymerase Chain Reaction -- Assembly of DNA Sequencing Data -- Sequence Finishing -- Quality Assessment of Finished BAC Sequences.
|
| 650 |
|
0 |
|a Bacteriology.
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| 650 |
|
0 |
|a Microbiology.
|
| 650 |
|
0 |
|a Biochemistry.
|
| 650 |
1 |
4 |
|a Bacteriology.
|
| 650 |
2 |
4 |
|a Microbiology.
|
| 650 |
2 |
4 |
|a Biochemistry, general.
|
| 700 |
1 |
|
|a Zhao, Shaying.
|e editor.
|
| 700 |
1 |
|
|a Stodolsky, Marvin.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|